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P4HB 蛋白, Human (His)

目录号: HY-P71917
COA 产品使用指南

P4HB 蛋白是一种多功能实体,可催化细胞表面和细胞内的二硫键键合过程。作为还原酶,它修饰外表面蛋白质并参与新生蛋白质中二硫键的形成。P4HB 蛋白, Human (His) 是重组的 P4HB 蛋白,由 E. coli 表达,带有 N-6*His 标签。P4HB 蛋白, Human (His) 全长 490 个氨基酸,分子量约为 ~56 kDa。

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描述

The P4HB protein is a multifunctional entity that catalyzes disulfide bonding processes at the cell surface and within cells. As a reductase, it modifies outer surface proteins and participates in the formation of disulfide bonds in nascent proteins. P4HB Protein, Human (His) is the recombinant human-derived P4HB protein, expressed by E. coli , with N-6*His labeled tag. The total length of P4HB Protein, Human (His) is 490 a.a., with molecular weight of ~56 kDa.

研究背景

P4HB 蛋白是一种多功能实体,可催化二硫键形成、断裂和重排的复杂过程。它位于细胞表面,充当还原酶,裂解与细胞相连的蛋白质的二硫键,并可能导致外表面蛋白质的结构修饰。在细胞内,P4HB 参与新生蛋白质二硫键的形成和重排。当浓度升高时,在 FAM20C 磷酸化的影响下,它充当伴侣,防止错误折叠的蛋白质聚集。相反,在较低浓度下,它通过促进聚集而表现出抗伴侣活性。P4HB 的多功能性延伸到它作为结构亚基参与多种酶,例如脯氨酰 4-羟化酶和微粒体三酰甘油转移蛋白 MTTP。此外,它作为 LGALS9 的受体,通过这种相互作用影响 Th2 T 辅助细胞表面的二硫键还原酶活性,改变质膜的氧化还原状态,并增强细胞迁移。这种多样化的功能强调了 P4HB 在细胞过程中的关键作用,包括蛋白质折叠、氧化还原调节和细胞迁移。

生物活性

1.Thiol Protein Reductase Activity is 0.001 /650nm/ min-2, determined by measuring the turbidityincrease at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag tim.
2. Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is 9.375 A650/cm/min/mg.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P07237 (A19-L508)

基因 ID
蛋白结构
N-term
6*His
P4HB (A19-L508)
Accession # P07237
C-term
同用名
Cellular thyroid hormone binding protein; Cellular thyroid hormone-binding protein; Collagen prolyl 4 hydroxylase beta; Disulphide Isomerase; DSI; EC 5.3.4.1; ER protein 59; ERBA2L; ERp59; GIT; P4HB; P4Hbeta; p55; PDI; PDIA1; PDIA1_HUMAN; PDIR; PHDB; PO4DB; PO4HB; PROHB; Protocollagen hydroxylase; Thbp;
氨基酸序列

APEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

分子量

Approximately 56 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

P4HB Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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