PDGF-BB 蛋白, Human

PDGF-BB Protein, Human is the most active PDGF isoform, binds to PDGF receptor, promotes diverse cell types proliferation and osteogenesis, and further stimulates bone formation in fracture or defect.

重组人血小板衍生生长因子-BB是最活跃的PDGF亚型，与PDGF受体结合，促进多种细胞类型增殖和成骨，并进一步刺激骨折或缺损处的骨形成。血小板源性生长因子-BB (PDGF-BB) 主要由血小板 α 颗粒分泌，是骨骼和其他结缔组织中最活跃的 PDGF 亚型，因为它可以与所有已知的 PDGF 受体结合。PDGF-BB 通过诱导有丝分裂、趋化性、细胞外基质形成和血管化在骨再生中发挥重要作用^[1]。重组人血小板衍生生长因子-BB (rhPDGF-BB) 通过改善基质重塑、增加胶原蛋白合成和增加细胞增殖来加速肌腱愈合。重组人血小板衍生生长因子-BB 在非破裂、退化肌腱病模型中也有效。此外，重组人血小板衍生生长因子-BB 通过诱导祖细胞和肌腱细胞的增殖和迁移来治疗慢性肌腱病，从而刺激退化肌腱的结构修复^[2]。

1.The ED₅₀ is <3 ng/mL as measured by murine Balb/c 3T3 cells, corresponding to a specific activity of >3.3 × 10⁵ units/mg.
2.Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED₅₀ for this effect is 5-42 ng/mL.

Human

E. coli

Tag Free

P01127 (S82-T190)

5155  [NCBI]

SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT

14-24.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM NaAc-HAc, pH 4.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sun H, et al. Recombinant human platelet-derived growth factor-BB versus autologous bone graft in foot and ankle fusion: A systematic review and meta-analysis. Foot Ankle Surg. 2017 Mar;23(1):32-39.  [Content Brief]

  [2]. Solchaga LA, et al. Comparison of the effect of intra-tendon applications of recombinant human platelet-derived growth factor-BB, platelet-rich plasma, steroids in a rat achilles tendon collagenase model. J Orthop Res. 2014 Jan;32(1):145-50.  [Content Brief]