1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E1 Enzymes
  5. PDHA1 蛋白, Human (His, GST)

PDHA1 蛋白, Human (His, GST)

目录号: HY-P704071
产品使用指南 技术支持

PDHA1蛋白是丙酮酸脱氢酶复合物的重要组成部分,负责协调丙酮酸向乙酰辅酶A和CO2的转化。它在糖酵解和三羧酸 (TCA) 循环之间建立了重要联系,确保代谢途径的无缝整合。PDHA1 蛋白, Human (His, GST) 是重组的 PDHA1 蛋白,由 E. coli 表达,带有 C-6*His 和 N-GST 标签。

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规格 是否有货
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100 μg   询价  

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描述

The PDHA1 protein is an important component of the pyruvate dehydrogenase complex and is responsible for coordinating the conversion of pyruvate to acetyl-CoA and CO2. It establishes an important link between glycolysis and the tricarboxylic acid (TCA) cycle, ensuring seamless integration of metabolic pathways. PDHA1 Protein, Human (His, GST) is the recombinant human-derived PDHA1 protein, expressed by E. coli, with C-6*His and N-GST labeled tag.

研究背景

PDHA1 蛋白是丙酮酸脱氢酶复合物的重要组成部分,在协调丙酮酸全面转化为乙酰辅酶 A 和 CO2 方面发挥着关键作用。这种酶促过程在糖酵解途径和三羧酸 (TCA) 循环之间建立了基本联系,促进代谢途径的无缝整合。PDHA1 的催化功能对于丙酮酸的有效转化至关重要,确保碳底物流入 TCA 循环,以实现细胞内随后的能量生成和代谢稳态。

种属

Human

表达系统

E. coli

标签

C-6*His;N-GST

蛋白编号

P08559 (A31-S390)

基因 ID

5160

蛋白结构
N-term
GST
A31-S390
Accession # P08559
6*His
C-term
蛋白长度

Partial

同用名
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1; PHE1A
氨基酸序列

ANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

预测分子量
71.6 kDa
纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PDHA1 Protein, Human (His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PDHA1 Protein, Human (His, GST)
目录号:
HY-P704071
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