1. 重组蛋白
  2. Enzymes & Regulators
  3. Carboxypeptidase
  4. Carboxypeptidase Q
  5. PGCP 蛋白, Mouse (HEK293, His)

PGCP 蛋白, Mouse (HEK293, His)

目录号: HY-P71200
产品使用指南

PGCP 是一种羧肽酶,在循环肽的水解中具有潜在的关键作用。它催化末端未取代的二肽的裂解,释放氨基酸。PGCP 蛋白, Mouse (HEK293, His) 是重组的 PGCP 蛋白,由 HEK293 表达,带有 C-6*His 标签。PGCP 蛋白, Mouse (HEK293, His) 全长 452 个氨基酸,分子量约为 ~60.0 kDa。

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描述

PGCP is a carboxypeptidase with a potentially critical role in the hydrolysis of circulating peptides. It catalyzes the cleavage of terminally unsubstituted dipeptides, releasing amino acids. PGCP Protein, Mouse (HEK293, His) is the recombinant mouse-derived PGCP protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PGCP Protein, Mouse (HEK293, His) is 452 a.a., with molecular weight of ~60.0 kDa.

研究背景

PGCP(肽聚糖识别蛋白 2)是一种羧肽酶,在循环肽的水解中具有潜在的关键作用。这种酶催化末端未取代的二肽的水解,将它们分解成单个氨基酸。有建议认为 PGCP 可能参与甲状腺素激素从甲状腺球蛋白前体的释放,表明其可能参与甲状腺激素调节。在结构上,PGCP 以同型二聚体形式存在,单体形式无活性,而同型二聚体构型则表现出酶活性。这种二聚体结构表明了 PGCP 激活的调节机制,强调了其在循环肽加工和代谢中的重要性。

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9WVJ3 (K19-S470)

基因 ID

54381  [NCBI]

蛋白结构
N-term
PGCP (K19-S470)
Accession # Q9WVJ3
6*His
C-term
同用名
PGCP; Carboxypeptidase Q; Hematopoietic lineage switch 2; Plasma glutamate carboxypeptidase; Cpq; Hls2
氨基酸序列

KAVFKNGVSQRTFREIKEEIANYEDVAKAIINLAVYGKYQNRSYERLGLLVDTVGPRLSGSKNLEKAIQIMYQNLQQDGLENVHLEQVRIPHWERGEESAVMLEPRIHKMAILGLGSSIGTPPGGITAEVLVVASFDELQRRASEARGKIIVYNQPYTGYEKTVQYRVQGAVEAAKVGAVASLIQSVASFSIYSPHTGIQKYQDGVPKIPTACITVEDAEMMSRMASRGNKIVIHLEMGAKTYPDTDSFNTVAEITGSMYPEEVVLVSGHLDSWDVGQGALDDGGGAFISWEALSLVKDLGLRPKRTLRLVLWTAEEQGGIGASQYYELHKANISKYSLVMEADSGTFLPTGLQFTGSDKARAIMKEVMNLLQPLNVTKVFSNGEGTDINFWIQAGVPGASLRDDLYKYFFFHHSHGDTMTVMDPKQMNVAAAVWAVVAYVVADMDEMLPRS

分子量

Approximately 60.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PGCP Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PGCP Protein, Mouse (HEK293, His)
目录号:
HY-P71200
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