PRDX4 蛋白, Human (His)

PRDX4, a thiol-specific peroxidase, enzymatically reduces hydrogen peroxide and organic hydroperoxides, crucial for cellular protection. It detoxifies peroxides, acts as a sensor for hydrogen peroxide-mediated signaling, and contributes to NF-kappa-B activation regulation. PRDX4's multifaceted activity underscores its significance in cellular redox homeostasis and potential impact on intracellular signaling. PRDX4 Protein, Human (His) is the recombinant human-derived PRDX4 protein, expressed by E. coli , with N-6*His labeled tag.

PRDX4 是一种硫醇特异性过氧化物酶，在过氧化氢和有机氢过氧化物分别还原为水和醇的过程中充当酶催化剂。它在细胞保护中的关键作用包括对过氧化物进行解毒并充当过氧化氢介导的信号事件的传感器。此外，PRDX4 通过调节 I-kappa-B-alpha 的磷酸化，有助于调节细胞质中 NF-kappa-B 的激活。这种多方面的活性强调了 PRDX4 在细胞氧化还原稳态中的重要性及其对细胞内信号通路的潜在影响。

Defined as the amount of hydroperoxide that 1μg of PRDX4 can reduce at 25°C for 1 minute. The specific activity is 1875- 2063.33 pmol/min/μg, as measured under the described conditions.

Materials
30% H₂O₂
PRDX4 Protein, Human (His) (HY-P71232)

Procedure
1. Dilute 30% hydrogen peroxide to concentrations of 0.3058, 0.1529, 0.076, 0.038, 0.019, 0.0096, 0.0047, 0 mol/L. Add 100 µL/well and measure the absorbance at 240 nm.
2. Dilute H₂O₂ to 0.2 mol/L.
3. Dilute the Human PRDX4 to 1000 µg/mL.
4. In a 96-well plate, add 90 µL of 0.2 mol/L H₂O₂ and 10 µL of 1000 µg/mL protein.
5. Read the initial absorbance at 240 nm.
6. Incubate at room temperature for 20 minutes and read the absorbance again.
7. Calculate specific activity:

Human

E. coli

N-6*His

Q13162 (W38-N271)

10549  [NCBI]

Full Length of Mature Protein

WETEERPRTREEECHFYAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVIDGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRLEEFRSINTEVVACSVDSQFTHLAWINTPRRQGGLGPIRIPLLSDLTHQISKDYGVYLEDSGHTLRGLFIIDDKGILRQITLNDLPVGRSVDETLRLVQAFQYTDKHGEVCPAGWKPGSETIIPDPAGKLKYFDKLN

27-30 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.