PRMT3 蛋白, Human (His)

PRMT3 protein is a multifunctional protein arginine N-methyltransferase. As a type I methyltransferase, it catalyzes monomethylation and asymmetric dimethylation of arginine residues in target proteins. In addition to its methyltransferase role, PRMT3 may also regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. PRMT3 Protein, Human (His) is the recombinant human-derived PRMT3 protein, expressed by E. coli , with N-10*His labeled tag. The total length of PRMT3 Protein, Human (His) is 530 a.a., with molecular weight of ~65 kDa.

PRMT3 蛋白是一种多功能蛋白精氨酸 N-甲基转移酶，催化靶蛋白中精氨酸残基的胍基氮的单甲基化和不对称二甲基化，将其归类为 I 型甲基转移酶。除了作为甲基转移酶的作用外，PRMT3 还通过抑制 ALDH1A1 视网膜脱氢酶活性来潜在地调节视黄酸合成和信号转导，强调了它在调节关键细胞过程中的作用。PRMT3 的双重酶活性强调了其在蛋白质翻译后修饰中的重要性，并表明其对多种生物途径的潜在影响，特别是与视黄酸代谢和信号传导相关的途径。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-10*His

O60678-1 (C2-Q531)

10196  [NCBI]

CSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ

Approximately 63 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.