Prostatic acid phosphatase/ACPP 蛋白, Human (354a.a, HEK293, His, solution)

PPAP protein, a nonspecific tyrosine phosphatase, has anticancer and antiproliferative activities. The PPAP protein dephosphorylates a variety of substrates under acidic conditions (pH 4-6), resulting in the inactivation of lysophosphatidic acid in seminal plasma. The PPAP protein is also a common biomarker for prostate cancer and can inhibit tumor development. Prostatic acid phosphatase/ACPP Protein, Human (354a.a, HEK293, His, solution) is the recombinant human-derived Prostatic acid phosphatase/ACPP protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Prostatic acid phosphatase/ACPP Protein, Human (354a.a, HEK293, His, solution) is 354 a.a., with molecular weight of ~50.0 kDa.

PPAP 蛋白 (cPAcP)，是一种非特异性酪氨酸磷酸酶和前列腺上皮分化抗原，参与调节前列腺上皮的生长。PPAP 可在酸性条件 (pH 4-6) 下使多种底物去磷酸化，并导致精浆中的溶血磷脂酸失活。它主要在 Tyr(1221/2) 处与 ErbB-2 相互作用并使 ErbB-2 去磷酸化，从而阻断下游信号传导，导致细胞生长减少。它还部分通过 ErbB-2 的去磷酸化发挥前列腺癌 (PCa) 细胞负生长调节剂的作用。PPAP 蛋白常作为前列腺癌的生物标志物。通过 ERBB2 去磷酸化和 MAPK 介导的信号传导失活而抑制前列腺癌发展。因而，雄激素非依赖性细胞增殖和致瘤性的发生，与细胞内 PPAP 蛋白表达降低紧密相关。肿瘤内注射 PAcP cDNA 表达载体可抑制异种移植肿瘤进展并降低 ErbB-2 酪氨酰磷酸。在 cPAcP 降低的前列腺癌和 PCa 细胞中，cPAcP 降低导致 ErbB-2 和 ERK1/2 信号转导激活。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAH16344.1 (K33-D386)

55  [NCBI]

KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQGTEDSTD

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.