SCF 蛋白, Human

SCF proteins are ligands for the KIT receptor-type protein tyrosine kinase and regulate a variety of cellular processes, including survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Binding to KIT activates signaling pathways involving PIK3R1, AKT1, GRB2, RAS, RAF1, MAP kinase, STAT family members, and PLCG1, producing diacylglycerol and inositol 1,4,5-trisphosphate. SCF Protein, Human is the recombinant human-derived SCF protein, expressed in E. coli.

干细胞因子（SCF）也称为肥大细胞生长因子（MGF）和钢因子（SLF），在配子发生、黑素生成和造血早期阶段发挥重要作用。SCF是一种二聚体分子，通过结合并激活受体酪氨酸激酶c-Kit^[1][2]来发挥其生物学功能。

SCF Protein, Human (0-110 ng/mL, 7-42 days) 是 c-kit 原癌基因的配体，可促进早期造血祖细胞的增殖和分化，以及多种细胞类型的生长和分化。SCF Protein, Human 可促进人胎肝细胞分化为肥大细胞^[3]。
SCF Protein, Human (0-100 ng/mL, 72 h) 可提高人红白血病细胞 TF-1 的细胞活力，并防止细胞死亡^[4]。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL, corresponding to a specific activity is > 2×10^5 units/mg.

• 
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.194 ng/mL, corresponding to a specific activity is 8.38×10⁵ units/mg.

Human

E. coli

Tag Free

P21583-1 (E26-A189)

4254  [NCBI]

Full Length of Soluble KIT ligand

EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA

Approximately 19 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0 or 50 mM Tris-HCL, 300 mM NaCL, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Dehbashi M, Kamali E, Vallian S. Comparative genomics of human stem cell factor (SCF). Mol Biol Res Commun. 2017;6(1):1-11.  [Content Brief]

  [2]. Lennartsson J, Rönnstrand L. Stem cell factor receptor/c-Kit: from basic science to clinical implications. Physiol Rev. 2012;92(4):1619-1649.  [Content Brief]

  [3]. Irani A M, et al. Recombinant human stem cell factor stimulates differentiation of mast cells from dispersed human fetal liver cells[J]. 1992.

  [4]. Akuta T, et al., Expression of bioactive soluble human stem cell factor (SCF) from recombinant Escherichia coli by coproduction of thioredoxin and efficient purification using arginine in affinity chromatography. Protein Expr Purif. 2015 Jan;105:1-7.  [Content Brief]