Semaphorin-3A/SEMA3A 蛋白, Mouse (HEK293, Fc)

Semaphorin-3A/SEMA3A Protein guides growth cones and is implicated in sensory projection patterning, selectively repelling axons that terminate dorsally. It contributes to olfactory system development and neuronal control of puberty. SEMA3A interacts with PXND1, although the exact nature of this interaction is unspecified. Semaphorin-3A/SEMA3A Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Semaphorin-3A/SEMA3A protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Semaphorin-3A/SEMA3A Protein, Mouse (HEK293, Fc) is 521 a.a., with molecular weight of 80-95 kDa.

Semaphorin-3A/SEMA3A 蛋白在引导生长锥中发挥着至关重要的作用。据信，它通过选择性地排斥通常终止于背侧的轴突来参与感觉投射的模式化。SEMA3A 还参与嗅觉系统的发育，并有助于青春期的神经元控制。此外，它与 PXND1 相互作用，尽管这种相互作用的确切性质尚未明确。

Measured by its binding ability in a functional ELISA. Immobilized Human Neuropilin-1 at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A. The ED₅₀ for this effect is 41.82-166 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human Neuropilin-1 at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A.The ED₅₀ for this effect is 165.6 ng/mL.

Mouse

HEK293

N-hFc

O08665 (K26-F546)

20346  [NCBI]

KNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLEAYNQTHLYACGTGAFHPICTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRPIMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEILLEEMTVFREPTTISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYF

Approximately 80-95 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.