1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Heat Shock Protein 47
  6. Serpin H1 蛋白, Human (HEK293, His)

Serpin H1 蛋白, Human (HEK293, His)

目录号: HY-P71298
COA 产品使用指南

Serpin H1 蛋白对胶原蛋白表现出特异性亲和力,表明其在胶原蛋白生物合成途径中作为伴侣的作用。这表明它对胶原蛋白合成的潜在贡献,强调了它在维持组织完整性和调节与胶原蛋白代谢相关的细胞反应方面的重要性。Serpin H1 蛋白, Human (HEK293, His) 是重组的 丝氨酸蛋白酶抑制剂 H1 蛋白,由 HEK293 表达,带有 C-6*His 标签。Serpin H1 蛋白, Human (HEK293, His) 全长 400 个氨基酸,分子量约为 ~51.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥3900 In-stock
100 μg   询价  

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描述

Serpin H1 protein exhibits a specific affinity for collagen, indicating its role as a chaperone in the biosynthetic pathway of collagen. This suggests its potential contribution to collagen synthesis, emphasizing its importance in maintaining tissue integrity and modulating cellular responses related to collagen metabolism. Serpin H1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin H1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin H1 Protein, Human (HEK293, His) is 400 a.a., with molecular weight of ~51.0 kDa.

研究背景

Serpin H1 蛋白表现出对胶原蛋白的特异性亲和力,表明其在选择性结合这种细胞外基质蛋白方面的作用。这种相互作用表明 Serpin H1 可能在胶原蛋白生物合成途径中发挥伴侣分子的功能,可能有助于复杂的胶原蛋白合成过程。由于其与胶原蛋白的特异性结合,Serpin H1 可能参与调节胶原蛋白相关的生物过程,强调其在维持组织结构完整性和调节与胶原蛋白代谢相关的细胞反应方面的潜在重要性。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P50454 (A19-L418)

基因 ID

871  [NCBI]

蛋白结构
N-term
Serpin H1 (A19-L418)
Accession # P50454
6*His
C-term
同用名
Serpin H1; 47 kDa Heat Shock Protein; Arsenic-Transactivated Protein 3; AsTP3; Cell Proliferation-Inducing Gene 14 Protein; Collagen-Binding Protein; Colligin; Rheumatoid Arthritis-Related Antigen RA-A47; SERPINH1; CBP1; CBP2; HSP47; SERPINH2
氨基酸序列

AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

分子量

Approximately 51.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Serpin H1 Protein, Human (HEK293, His)
目录号:
HY-P71298
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