Siglec-10 蛋白, Human (Biotinylated, R119A, HEK293, His-Avi)

Siglec-10 Protein, identified as a putative adhesion molecule, mediates sialic-acid dependent cellular binding, preferring alpha-2,3- or alpha-2,6-linked sialic acid. Its sialic acid recognition site may undergo masking through cis interactions. In immune responses, Siglec-10 acts as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation, recruiting SH2 domain-containing phosphatases, and negatively regulating B-cell antigen receptor signaling via PTPN6/SHP-1. In collaboration with CD24, it selectively suppresses the immune response to DAMPs, regulates NK cell response, controls autoimmunity, and inhibits cross-presentation during adaptive immune responses by CD8-alpha(+) dendritic cells. Siglec-10 interacts with proteins including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11. Siglec-10 Protein, Human (Biotinylated, R119A, HEK293, His-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Siglec-10 Protein, Human (Biotinylated, R119A, HEK293, His-Avi) is 530 a.a., with molecular weight of 68-80 kDa.

Siglec-10 蛋白被认为是一种推定的粘附分子，在唾液酸依赖性细胞结合中发挥作用，显示出对 α-2,3- 或 α-2,6- 连接唾液酸的偏好。 Siglec-10 的唾液酸识别位点可能由于与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。在免疫反应中，它似乎充当抑制性受体，诱导配体诱导的酪氨酸磷酸化并通过其 SH2 结构域招募细胞质磷酸酶，通过信号分子的去磷酸化来阻断信号转导。 Siglec-10 依赖于 PTPN6/SHP-1，参与 B 细胞抗原受体信号传导的负向调节。与 CD24 结合，它可能参与选择性抑制对危险相关分子模式 (DAMP)（例如 HMGB1、HSP70 和 HSP90）的免疫反应。 Siglec-10 与 CD24 协同调节自然杀伤 (NK) 细胞的免疫反应，并在控制自身免疫方面发挥作用。在 CD8-α(+) 树突状细胞启动适应性免疫反应期间，Siglec-10 通过损害 MHC I 类肽复合物的形成来抑制交叉呈递，从而招募 PTPN6/SHP-1 并促进吞噬体酸化。 Siglec-10 与多种蛋白质相互作用，包括 PTPN6/SHP-1、NCF1、CD24、HMGB1、RIGI、CBL 和 PTPN11。

1.Immobilized Anti-Siglec-10 Antibody hFc at 2 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) His with the EC₅₀ of 74.8 μg/mL determined by ELISA.
2.Immobilized Anti-Siglec-10 Antibody hFc at 5 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) His with the EC₅₀ of 61.7 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q96LC7-1 (M17-T546)

89790  [NCBI]

MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLIST

68-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 200 mM L-Arginine, pH 8.0. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.