1. 重组蛋白
  2. Receptor Proteins
  3. Siglec
  4. Siglec-15
  5. Siglec-15 蛋白, Cynomolgus (HEK293, His)

Siglec-15 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P70743
COA 产品使用指南

Siglec-15 蛋白是 Siglec 家族的成员和 1 型跨膜蛋白,在破骨细胞、巨噬细胞和树突细胞中组成型表达。Siglec-15 在破骨细胞生成的发育和分化中发挥着关键作用,在体外和体内抑制抗原特异性 T 细胞反应。Siglec-15 蛋白, Cynomolgus (HEK293, His) 是重组的 Siglec-15 蛋白,由 HEK293 表达,带有 C-6*His 标签。Siglec-15 蛋白, Cynomolgus (HEK293, His) 全长 244 个氨基酸,分子量为 30-40 kDa。

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10 μg ¥800 In-stock
50 μg ¥2400 In-stock
100 μg   询价  

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描述

Siglec-15 Protein, a Siglec family member and type-1 transmembrane protein, is constitutively expressed in osteoclasts, macrophages and dendritic cells. Siglec-15 plays a pivotal role in the development and differentiation of osteoclastogenesis, inhibiting antigen-specific T cell responses in vitro and in vivo. Siglec-15 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-15 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-15 Protein, Cynomolgus (HEK293, His) is 244 a.a., with molecular weight of 30-40 kDa.

研究背景

Siglec-15是 Siglec 家族的成员和 1 型跨膜蛋白,在破骨细胞、巨噬细胞和树突状细胞中组成型表达。Siglec-15 在肌动蛋白细胞骨架组织的上游或调控范围内发挥作用。Siglec-15缺失可以促进骨形成并减少骨吸收,表明Siglec-15在破骨细胞生成的发育和分化中起着关键作用,可能作为抑制骨吸收和促进骨量增加的骨重塑的靶点。Siglec-15 是一种主要由巨噬细胞介导的 T 细胞反应抑制剂。在肿瘤中,Siglec-15 受到 IFN-γ 的负向调节,从而影响效应 T 细胞介导的抗肿瘤免疫。Siglec-15 的基因消融或抗体阻断可增强 TME 中的抗肿瘤免疫力,并抑制某些小鼠模型中的肿瘤生长。Siglec-15 可作为正常化癌症免疫治疗的潜在靶点[1][2][3][4]

种属

Cynomolgus

表达系统

HEK293

标签

C-6*His

蛋白编号

A0A2K5UY47 (F20-T263)

基因 ID

/

蛋白结构
N-term
Siglec-15 (F20-T263)
Accession # A0A2K5UY47
6*His
C-term
同用名
Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3
氨基酸序列

FVRTKIDTTENLLNTEVHSSPAQRWSMQVPAEVSAAAGDAAVLPCTFTHPHRHYDGPLTAIWRAGEPYAGPQVFRCAAARGSELCQTALSLHGRFRLLGNPRRNDLSLRVERLALADDRRYFCRVEFAGDVHDRYESRHGVRLHVTAAPRIINISVLPGPAHAFRALCTAEGEPPPALAWSGPALGNGSAAVPSSGQGHGHLVTAELPALNHDGRYTCTAANSLGRSEASVYLFRFHGASGAST

分子量

30-40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 150 mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Siglec-15 Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Siglec-15 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P70743
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