MAG/Siglec-4a 蛋白, Mouse (HEK293, His)

MAG/Siglec-4a protein mediates myelin cell-neuron interactions by binding to gangliosides, RTN4R, and RTN4RL2. It maintains normal axonal myelination and protects motor neurons from apoptosis following injury. MAG/Siglec-4a Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAG/Siglec-4a Protein, Mouse (HEK293, His) is 497 a.a., with molecular weight of 70-120 kDa.

MAG/Siglec-4a 蛋白是一种粘附分子，在介导髓鞘细胞和神经元之间的相互作用中发挥着至关重要的作用。它通过与含有唾液酸的神经节苷脂以及糖蛋白 RTN4R 和 RTN4RL2 结合来实现这一点。虽然它对于初始髓鞘形成不是必需的，但它参与维持正常的轴突髓鞘形成。此外，它可能通过与神经元 RTN4R 和 RTN4RL2 的相互作用，提供针对运动神经元凋亡的保护，特别是在损伤后。在成人中，它对于防止有髓轴突变性至关重要，并且该过程可能依赖于它与轴突细胞膜上的神经节苷脂的结合。此外，MAG/Siglec-4a 蛋白通过抑制轴突纵向生长，充当神经突生长的负调节因子。其对神经突延伸的抑制作用主要通过其与神经元 RTN4R、RTN4RL2 和神经节苷脂的相互作用来介导。它优先与 α-2,3-连接的唾液酸和神经节苷脂 Gt1b 结合。MAG/Siglec-4a 蛋白可以单体或同二聚体的形式存在，并通过其前三个 N 端 Ig 样结构域与 BSG 的亚型 2 相互作用。

Measured  by its ability to inhibit SH-SY5Y growth. The ED₅₀ for this effect is 3.129  μg/mL, corresponding to a specific activity is 319.59 units/mg.

Mouse

HEK293

C-6*His

P20917 (G20-P516)

17136  [NCBI]

GHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

70-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES,150 mM NaCl,1 mM EDTA, pH 7.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.