1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a 蛋白, Human (P78324, HEK293, His)

SIRP alpha/CD172a 蛋白, Human (P78324, HEK293, His)

目录号: HY-P7405
产品使用指南

SIRP alpha/CD172a 蛋白, Human (P78324, HEK293, His)在 HEK293 细胞中产生,具有 C-端6× His标签。SIRPα 是一种单体,约 90kDa 的 I 型跨膜糖蛋白,属于免疫球蛋白超家族的 SIRP/SHPS (CD172) 家族。

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10 μg ¥1900 询问价格 & 货期
50 μg ¥3900 询问价格 & 货期

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描述

SIRP alpha/CD172a Protein, Human (P78324, HEK293, His) is a polypeptide chain amino acids with the C-termimal His tag. Signal regulatory protein alpha (SIRPα, designated CD172a) is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily.

研究背景

Signal regulatory protein α (SIRPα) is a glycoprotein receptor that recruits and signals via the tyrosine phosphatases SHP-1 and SHP-2.The cytoplasmic domain of SIRPα contains four immunoreceptor tyrosine-based inhibition motifs (ITIMs), which upon phosphorylation recruit and activate SH2-domain-containing phosphotyrosine phosphatases (PTPase) SHP-1 and SHP-2. In macrophages SIRPα can negatively regulate the phagocytosis of host cells and the production of tumor necrosis factor alpha. SIRPα ligation induces macrophage NO production through the cooperative action of JAK/STAT and PI3-K/Rac1/NOX/H2O2 signaling pathways. It proposes that SIRPα may function as an inhibitory receptor[1].

生物活性

2 µg/mL (100 µL/well) of immoblized recombinant human Signal regulatory protein alpha-His can bind human CD47-Fc with a linear range of 0.25-185 ng/mL.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P78324 (E31-R370)

基因 ID

140885  [NCBI]

中文名
重组人信号调节蛋白,His (HEK 293表达)
同用名
rHuSignal regulatory protein α, His; CD172a; SHPS-1; BIT; MYD1
氨基酸序列

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERHHHHHH

分子量

50-55 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SIRP alpha/CD172a Protein, Human (P78324, HEK293, His)
目录号:
HY-P7405
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