1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a 蛋白, Rat (HEK293, His)

SIRP alpha/CD172a 蛋白, Rat (HEK293, His)

目录号: HY-P76073
COA 产品使用指南

当与 KLRD1 配对时,NKG2C/CD159c 蛋白可识别装载有 MHC-G 肽的 MHC-E,从而有助于 NK 细胞的生成和功能。它还调节病毒感染中的细胞毒性淋巴细胞。SIRP alpha/CD172a 蛋白, Rat (HEK293, His) 是重组的 SIRP α/CD172a 蛋白,由 HEK293 表达,带有 C-His 标签。SIRP alpha/CD172a 蛋白, Rat (HEK293, His) 全长 342 个氨基酸,分子量为 63-80 kDa。

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描述

When paired with KLRD1, the NKG2C/CD159c protein recognizes MHC-E loaded with MHC-G peptides, thereby contributing to NK cell generation and function. It also regulates cytotoxic lymphocytes in viral infections. SIRP alpha/CD172a Protein, Rat (HEK293, His) is the recombinant rat-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-His labeled tag. The total length of SIRP alpha/CD172a Protein, Rat (HEK293, His) is 342 a.a., with molecular weight of 63-80 kDa.

研究背景

SIRP α/CD172a 蛋白是 CD47 的免疫球蛋白样细胞表面受体,在各种细胞过程中充当多功能介质。作为对接蛋白,它诱导 PTPN6、PTPN11 和其他结合配偶体从细胞质易位到质膜。除了在细胞粘附中的作用外,SIRP α 还支持小脑神经元的粘附、神经突生长和神经胶质细胞附着。此外,它在突触发生和突触功能期间的细胞内信号传导中发挥着潜在的关键作用。SIRP α 参与负调节,调节由细胞粘附、生长因子或胰岛素诱导的受体酪氨酸激酶偶联细胞反应。它进一步介导吞噬作用、肥大细胞激活和树突细胞激活的负调节。值得注意的是,CD47 结合可防止未成熟树突细胞的成熟,并抑制成熟树突细胞产生细胞因子。SIRP α 的重要性延伸到抗病毒免疫,因为它通过减少病毒内化来限制新世界沙粒病毒感染。作为 THBS1 的受体,它刺激 SIRPA 的磷酸化,并响应 THBS1,参与非吞噬细胞中的 ROS 信号传导,刺激 NADPH 氧化酶衍生的 ROS 产生。SIRP α 与 PTPN11、GRB2、FGR、JAK2、SCAP1、SCAP2、FYB1、PTK2B 和 TRIM2 等蛋白质进行多种相互作用,说明其在细胞信号传导和免疫反应中作为核心参与者的复杂作用。

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD47 is present at 5 μg/mL, can bind Biotinylated Recombinant Rat CD172a. The ED50 for this effect is 157.1 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD47 is present at 5 μg/mL, can bind Biotinylated Recombinant Rat CD172a .The ED50 for this effect is 157.1 ng/mL.
种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P97710 (K32-N373)

基因 ID

25528  [NCBI]

蛋白结构
N-term
CD172a (K32-N373)
Accession # P97710
His
C-term
同用名
Signal-regulatory protein alpha; CD172a; SIRP alpha; SIRPA; MFR; SHPS1; SIRP
氨基酸序列

KELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWN

分子量

63-80 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SIRP alpha/CD172a Protein, Rat (HEK293, His)
目录号:
HY-P76073
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