1. 重组蛋白
  2. Enzymes & Regulators
  3. Protein Tyrosine Kinases
  4. Src 蛋白, Mouse (sf9, His-GST)

Src 蛋白, Mouse (sf9, His-GST)

目录号: HY-P73423
COA 产品使用指南

Src 是一种非受体蛋白酪氨酸激酶,具有以高亲和力磷酸化突触素的独特能力。Src 蛋白, Mouse (sf9, His-GST) 是重组的 Src 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。Src 蛋白, Mouse (sf9, His-GST) 全长 535 个氨基酸,分子量约为 ~80 kDa。

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规格 价格 是否有货 数量
10 μg ¥1150 In-stock
50 μg ¥3200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

Src Protein, Mouse (sf9, His-GST) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Src, a non-receptor protein tyrosine kinase, displays a unique ability to phosphorylate synaptophysin with high affinity. Src Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Src protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Src Protein, Mouse (sf9, His-GST) is 535 a.a., with molecular weight of ~80 kDa.

研究背景

Src 是一种非受体蛋白酪氨酸激酶,具有高亲和力磷酸化突触素的独特能力。

生物活性

1.The specific activity was determined to be > 50 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
2.Measured by its binding ability in a functional ELISA. Immobilized mouse SRC at 2 μg/mL (100 μL/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/mL.

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

P05480-2 (M1-L535)

基因 ID
蛋白结构
N-term
His-GST
Src (M1-L535)
Accession # P05480-2
C-term
同用名
Proto-oncogene tyrosine-protein kinase Src; Src; p60-Src; SRC1
分子量

Approximately 80 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Src Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Src Protein, Mouse (sf9, His-GST)
目录号:
HY-P73423
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