TGFBR2/TGF-beta RII 蛋白, Human (sf9, His)

The TGFBR2/TGF-β RII protein cooperates with TGFBR1 to form dedicated receptors for TGFB1, TGFB2, and TGFB3. It serves as a signal transducer that coordinates diverse physiological and pathological processes, including cell cycle regulation, mesenchymal cell dynamics, wound healing, matrix production, immunosuppression, and carcinogenesis. TGFBR2/TGF-beta RII Protein, Human (sf9, His) is the recombinant human-derived TGFBR2/TGF-beta RII protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of TGFBR2/TGF-beta RII Protein, Human (sf9, His) is 144 a.a., with molecular weight of ~24 kDa.

跨膜丝氨酸/苏氨酸激酶 TGFBR2 (TGF-β RII) 与 TGF-β I 型丝氨酸/苏氨酸激酶受体 TGFBR1 协作，形成 TGF-β 细胞因子的专用受体，包括 TGFB1、TGFB2 和 TGFB3。TGFBR2 作为信号转导器，介导 TGFB1、TGFB2 和 TGFB3 信号从细胞表面到细胞质的传递，从而协调多种生理和病理过程。这些包括上皮细胞和造血细胞的细胞周期停滞、间充质细胞增殖和分化的调节、伤口愈合、细胞外基质的产生、免疫抑制和致癌作用。该受体复合物包含与细胞因子二聚体对称结合的 2 个 TGFBR1 和 2 个 TGFBR2 分子，导致 TGFBR1 被组成型活性 TGFBR2 磷酸化和激活。激活的 TGFBR1 随后磷酸化 SMAD2，导致其与受体解离并与 SMAD4 相互作用。由此产生的 SMAD2-SMAD4 复合物易位到细胞核，在细胞核中调节 TGF-β 调节基因的转录，构成典型的 SMAD 依赖性 TGF-β 信号级联。此外，TGFBR2 参与非经典、不依赖 SMAD 的 TGF-β 信号传导途径，并表现出转化生长因子 β 激活受体活性。

Measured by its binding ability in a functional ELISA. Immobilized TGFBR2 at 10 μg/mL (100 μL/well) can bind TGFB1-His and the EC₅₀ is 130-300 ng/mL.

Human

Sf9 insect cells

C-His

P37173 (T23-Q166)

7048  [NCBI]

TIPPHVQKSVNNDMIVTDNNGAVKFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDPKLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLVIFQ

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM PBS, 150 mM NaCl, 10 % glycerol, 0.5 mM TCEP, pH 7.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.