TMEM175 蛋白, Human (HEK293)

TMEM175 Protein, Human (HEK293) is the recombinant human-derived TMEM175, expressed by HEK293 , with tag Free labeled tag. ,

TMEM175 是一种质子激活的质子通道 (proton-activated proton channel)，主要催化质子从内体 (endosomes) 和溶酶体 (lysosomes) 外流以维持稳态 pH 值。它在低 pH 条件下 (低于 4.6) 被激活，选择性介导溶酶体质子释放，平衡 V-ATPase 活性以维持 pH 稳态 (by similar: PubMed:35750034)。此外，TMEM175 在高 pH 时也作为钾通道 (potassium channel) 发挥作用，调节内体和溶酶体的钾电导。它构成 lysoK(GF) 复合物的孔形成亚基 (pore-forming subunit)，该复合物由 TMEM175 和 AKT (AKT1、AKT2 或 AKT3) 组成，受细胞外生长因子激活。lysoK(GF) 复合物通过 AKT 诱导的构象变化打开 TMEM175 通道，从而激活其功能，并在神经元应激损伤保护中起关键作用 (by similar: PubMed:33505021)。

Human

HEK293

Tag Free

Q9BSA9-1 (M1-C504)

84286

Full Length of Isoform-1

Approximately 56.3 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 8.0, 150 mM KCl, 2 mM DTT, 0.1% (w/v) LMNG.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.