TNF RII/TNFRSF1B 蛋白, Human (183a.a)

The TNFRII protein is a receptor with high affinity for TNFSF2/TNF-α and low affinity for TNFSF1/lymphotoxin-α, and plays a key role in mediating the metabolic effects of TNF-α. The TRAF1/TRAF2 complex recruits BIRC2 and BIRC3 to TNFRSF1B/TNFR2 to regulate the apoptotic pathway. TNFRII Protein, Human (183a.a) is the recombinant human-derived TNFRII protein, expressed by E. coli , with tag free.

TNFRII 蛋白作为受体，对 TNFSF2/TNF-α 具有高亲和力，对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。TRAF1/TRAF2 复合物将细胞凋亡抑制因子 BIRC2 和 BIRC3 募集至 TNFRSF1B/TNFR2，从而调节细胞凋亡途径。该受体在介导 TNF-α 诱导的大部分代谢作用中发挥着关键作用。值得注意的是，其亚型 2 表现出阻断 TNF-α 诱导的细胞凋亡的能力，表明在拮抗 TNF-α 的生物活性中具有调节作用。此外，TNFRII 与 TRAF2 结合，突显其参与下游信号级联反应。此外，与 BMX 和 XPNPEP3 激活形式的相互作用进一步增加了其分子关联的复杂性。

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 0.125 μg/mL in the presence of 1.25 ng/mL of recombinant Human TNF-alpha. Corresponding to a specific activity is 8×10^3U/mg.

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  Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 0.125 μg/mL in the presence of 1.25 ng/mL of recombinant Human TNF-alpha. Corresponding to a specific activity is 8×10³U/mg.

Human

E. coli

Tag Free

P20333-1 (P24-T206)

7133  [NCBI]

Partial

PAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPT

Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

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  Greater than 95% as determined by reducing SDS-PAGE..

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.