TOP2A 蛋白, Human

TOP2A Protein, Human is the recombinant human-derived TOP2A protein, expressed by E. coli, with tag free tag.

TOP2A 是一种关键的 DNA 解链酶，通过结合两个双链 DNA 分子来改变 DNA 拓扑结构。其作用机制包括在一条 DNA 链上产生双链断裂，将完整的链穿过断裂的链，并重新连接断裂的链。此外，TOP2A 可能参与调节 BMAL1 转录振荡的周期长度 (By similarity)。

Human

E. coli

Tag Free

P11388-1 (S1354-D1473)

7153

Partial

SPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDD

Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 0.5 M KCl, pH8.0 with glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.