TPO/Thrombopoietin 蛋白, Mouse (CHO)

TPO Protein, Mouse (CHO) is a hematopoietic factor that controls platelet production.

重组血小板生成素可减少放射和化疗引起的血小板减少症，增强骨髓移植后的血小板恢复，并增加干细胞收获物中巨核细胞前体细胞的数量。向动物注射血小板生成素可刺激骨髓巨核细胞的数量、大小和倍性，并使血小板计数增加至十倍^[1]。超生理量的血小板生成素/TPO (>100 ng/mL) 能够在体外直接激活血小板聚集。血小板生成素/TPO 对血流下的血小板粘附也有显着影响。低血小板生成素/TPO 浓度 (001-1 ng/mL) 可加速血小板与血管性血友病因子的牢固粘附，并防止在较高流速下脱离^[2]。

The ED₅₀ is <2 ng /mL as measured by MO7e cells.

Mouse

CHO

Tag Free

P40226 (S22-T356)

21832  [NCBI]

SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET

Approximately 75 kDa, due to glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kuter DJ, et al. Thrombopoietin: Biology and Clinical Applications. Oncologist. 1996;1(1 & 2):98-106.  [Content Brief]

  [2]. Hitchcock IS, et al. Thrombopoietin from beginning to end. Br J Haematol. 2014 Apr;165(2):259-68.  [Content Brief]