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Transferrin 蛋白, Human (HEK293, His)

目录号: HY-P70620
COA 产品使用指南

转铁蛋白是铁结合转运蛋白,结合两个 Fe(3+) 离子和一个阴离子(通常是碳酸氢根)。它们将铁从吸收和血红素降解地点运输到储存和利用地点。血清转铁蛋白可能影响细胞增殖。在微生物感染中,奈瑟菌属物种利用转铁蛋白作为铁源,捕获和提取铁以满足其代谢需求。Transferrin 蛋白, Human (HEK293, His) 是重组的 转铁蛋白,由 HEK293 表达,带有 C-His 标签。Transferrin 蛋白, Human (HEK293, His) 全长 679 个氨基酸,分子量为 68-87 kDa。

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10 μg ¥570 In-stock
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100 μg ¥2000 In-stock
500 μg ¥5600 In-stock
1 mg ¥8200 In-stock
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描述

Transferrin proteins, iron-binding transporters, bind two Fe(3+) ions and an anion, usually bicarbonate. They transport iron from absorption and heme degradation sites to storage and utilization locations. Serum transferrin may impact cell proliferation. In microbial infection, Neisseria species utilize transferrin as an iron source, capturing and extracting iron for their metabolic needs. Transferrin Protein, Human (HEK293, His) is the recombinant human-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Transferrin Protein, Human (HEK293, His) is 679 a.a., with molecular weight of 68-87 kDa.

研究背景

转铁蛋白作为铁结合转运蛋白,能够将两个 Fe(3+) 离子与一个阴离子(通常是碳酸氢根)结合。它们在将铁从吸收和血红素降解部位运输到指定储存和利用部位方面发挥着关键作用。此外,血清转铁蛋白可能对细胞增殖产生影响。在微生物感染的情况下,转铁蛋白可作为奈瑟氏菌属物种的铁源,奈瑟氏菌属物种能够熟练地捕获蛋白质并提取铁以满足自身的代谢需求。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R. The ED50 for this effect is 10.21 ng/mL.
2. Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1817 μg/mL, corresponding to a specific activity is 5503.58 units/mg

  • Measured by its binding ability in a functional ELISA. Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R,The ED50  for this effect is 10.21 ng/mL
种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P02787/AAA61140.1/NP_001054.1 (V20-P698)

基因 ID
蛋白结构
N-term
Transferrin (V20-P698)
Accession # P02787
His
C-term
同用名
Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF; TRF
氨基酸序列

VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP

分子量

68-87 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Transferrin Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Transferrin Protein, Human (HEK293, His)
目录号:
HY-P70620
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