1. 重组蛋白
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Trk Receptors TrkB
  5. TrkB 蛋白, Human (HEK293, His)

TrkB 蛋白, Human (HEK293, His)

目录号: HY-P70793
COA 产品使用指南

TrkB 蛋白是一种关键受体酪氨酸激酶,在中枢和外周神经系统发育中发挥关键作用。它调节神经元的存活、增殖、迁移、分化、突触形成和可塑性。TrkB 蛋白, Human (HEK293, His) 是重组的 TrkB 蛋白,由 HEK293 表达,带有 C-6*His 标签。TrkB 蛋白, Human (HEK293, His) 全长 399 个氨基酸,分子量为 60-105 kDa。

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规格 价格 是否有货 数量
10 μg ¥500 In-stock
50 μg ¥1500 In-stock
100 μg   询价  

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描述

The TrkB protein is a key receptor tyrosine kinase that plays a key role in central and peripheral nervous system development. It regulates neuronal survival, proliferation, migration, differentiation, synapse formation, and plasticity. TrkB Protein, Human (HEK293, His) is the recombinant human-derived TrkB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TrkB Protein, Human (HEK293, His) is 399 a.a., with molecular weight of 60-105 kDa.

研究背景

TrkB 蛋白是一种受体酪氨酸激酶,在中枢和周围神经系统的发育和成熟中发挥着关键作用。它调节各种过程,包括神经元存活、增殖、迁移、分化、突触形成和可塑性。TrkB 作为 BDNF、NTF4 的受体,也具有结合 NTF3 的能力,但效率较低。配体结合后,TrkB 发生同二聚化、自磷酸化和激活。这会激活下游效应器,例如 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1,它们控制不同的信号级联。GRB2-Ras-MAPK 级联受 SHC1、FRS2、SH2B1 和 SH2B2 调节,参与神经元分化和神经突生长。Ras-PI3 激酶-AKT1 级联也由这些效应器控制,主要调节细胞生长和存活。TrkB 还在突触可塑性中发挥作用,并参与学习和记忆。通过 PLCG1,TrkB 激活 NF-Kappa-B 并促进与细胞存活相关的基因的转录。此外,TrkB 参与神经胶质细胞中中性粒细胞依赖性钙信号传导,并促进神经元和神经胶质细胞之间的通讯。总体而言,TrkB 在细胞存活和各种神经过程中发挥着至关重要的作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q16620 (C32-H430)

基因 ID
蛋白结构
N-term
TrkB (C32-H430)
Accession # Q16620
6*His
C-term
同用名
BDNF/NT-3 Growth Factors Receptor; GP145-TrkB; Trk-B; Neurotrophic Tyrosine Kinase Receptor Type 2; TrkB Tyrosine Kinase; Tropomyosin-Related Kinase B; NTRK2; TRKB
氨基酸序列

CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREH

分子量

60-105 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TrkB Protein, Human (HEK293, His)
目录号:
HY-P70793
需求量: