TSLP 蛋白, Human

TSLP Protein, Human is an epithelial cell-derived cytokine which plays key role in allergic inflammation.

胸腺基质淋巴细胞生成素 (TSLP) 是一种上皮细胞衍生的细胞因子，在皮肤、肠道、肺和胸腺中表达。TSLP 通过 TSLPR 发出信号，TSLPR 是 IL-7 受体 α 链 (IL-7Rα) 和 TSLP 受体链 (TSLPR) 的异二聚体。胸腺基质淋巴细胞生成素对树突状细胞 (DC) 的极化产生深远影响，以驱动 T 辅助细胞 (Th) 2 细胞因子的产生。它还直接促进 T 细胞增殖，以响应 T 细胞受体 (TCR) 的激活和 Th2 细胞因子的产生。胸腺基质淋巴细胞生成素还支持 B 细胞扩增和分化。胸腺基质淋巴细胞生成素进一步增强肥大细胞和 NKT 细胞产生 Th2 细胞因子^[1]。多种先天免疫细胞表达 TSLPR 并对 TSLP 做出反应。例如，胸腺基质淋巴细胞生成素可以增强肥大细胞、NKT 细胞和嗜酸性粒细胞的细胞因子产生。此外，胸腺基质淋巴细胞生成素最近被证明可以诱导嗜酸性粒细胞胞外陷阱 (EET)，即感染时释放的线粒体 DNA 有毒颗粒分子的挤出^[2]。

1.The ED₅₀ is <0.3 ng/mL as measured by murine BaF3 pro-B cells, corresponding to a specific activity of >3.3 × 10⁶ units/mg.
2.Immobilized Human TSLP at 10 μg/mL (100 μl/well) can bind Human TSLP R-Fc .The ED₅₀ of Human TSLP R-Fc is <0.35 μg/mL.

Human

E. coli

Tag Free

Q969D9-1 (Y29-Q159)

85480  [NCBI]

Full Length of Isoform-1 Mature Protein

YDFTNCDFEKIKAAYLSTISKDLITYMSGTKSTEFNNTVSCSNRPHCLTEIQSLTFNPTAGCASLAKEMFAMKTKAALAIWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLQGLWRRFNRPLLKQQ

Approximately 15.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 150 mM NaCl or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. He R, et al. Thymic stromal lymphopoietin. Ann N Y Acad Sci. 2010 Jan;1183:13-24.  [Content Brief]

  [2]. Ziegler SF, et al. The biology of thymic stromal lymphopoietin (TSLP). Adv Pharmacol. 2013;66:129-55.  [Content Brief]

  [3]. Liu YJ, et al. Thymic stromal lymphopoietin: master switch for allergic inflammation. J Exp Med. 2006 Feb 20;203(2):269-73.  [Content Brief]