TSPO 蛋白, Mouse (Cell-Free, His)

The TSPO protein was originally thought to be a peripheral benzodiazepine receptor that binds protoporphyrin IX and isoquinoline carboxamide. TSPO Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived TSPO protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of TSPO Protein, Mouse (Cell-Free, His) is 169 a.a., with molecular weight of 21.7 kDa.

TSPO（转运蛋白）具有结合原卟啉 IX 的能力，表明其在卟啉和血红素的运输中具有潜在作用。TSPO 最初被确定为外周型苯二氮卓受体，还可以结合异喹啉甲酰胺。此外，它在促进胆固醇跨线粒体膜转运方面发挥作用，这表明它参与脂质代谢。尽管其精确的生理功能存在争议，并且一些报告表明它对于类固醇激素生物合成可能不是必需的，但 TSPO 与各种蛋白质（如 TSPOAP1、MOST-1 和潜在的 STAR）相互作用，表明它参与多种细胞过程。

Mouse

E. coli Cell-free

N-10*His

P50637 (M1-E169)

12257

MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRLPE

21.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.