TXNDC17 蛋白, Human

TXNDC17 Protein is a novel 14-kDa disul-fide reductase of the TXN (thioredoxin) family. It has peroxidase activity and protein-disulfide reductase (NAD(P)) activity. TXNDC17 is involved in the TNF (tumor necrosis factor) signaling pathway. And it inhibits the pathways of NF-κB, mitogen-activated protein kinases, and apoptosis in cells stimulated with TNF-alpha. Furthermore, TXNDC17 is an efficient S-denitrosylase. TXNDC17 Protein, Human is the recombinant human-derived TXNDC17 protein, expressed by E. coli , with tag free. The total length of TXNDC17 Protein, Human is 123 a.a., with molecular weight of ~13.9 kDa.

含硫氧还蛋白结构域的蛋白 17 (TXNDC17) 是 TXN (硫氧还蛋白) 家族的一种新型 14-kDa 二硫键还原酶。它具有过氧化物酶活性和蛋白质二硫键还原酶 (NAD(P)) 活性。TXNDC17 参与 TNF (肿瘤坏死因子) 信号通路。TXNDC17 抑制核因子 kappaB (NF-kappaB)、丝裂原激活蛋白激酶和肿瘤坏死因子 α (TNF-α) 刺激的细胞中的细胞凋亡途径。另外，TXNDC17 是一种高效的 S-脱亚硝基酶，在催化 S-亚硝基化谷胱甘肽或 HEK293 细胞衍生的 S-亚硝基蛋白的 TrxR1 依赖性脱亚硝基化方面具有与 Trx1 相似的效率^[1][2]。

Human

E. coli

Tag Free

A0A140VJY7 (M1-D123)

84817  [NCBI]

MARYEEVSVSGFEEFHRAVEQHNGKTIFAYFTGSKDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFRKNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLFSED

Approximately 13.9 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.