UBE2D1 蛋白, Human (GST)

UBE2D1 Protein, crucial in cellular processes, accepts ubiquitin from the E1 complex, preferentially catalyzing 'Lys-48'-linked polyubiquitination in vitro, showcasing versatility. It mediates selective degradation of short-lived and aberrant proteins and participates in E6/E6-AP-induced p53/TP53 ubiquitination. UBE2D1 facilitates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6, and TRIM63/MURF1. It ubiquitinates STUB1-associated HSP90AB1 in vitro, highlighting involvement in cellular pathways. Lacking inherent specificity for any ubiquitin lysine residue, UBE2D1's dynamic nature in ubiquitin modification processes is notable. Its essential role in viral activation of IRF3 and mediation of CYP3A4 polyubiquitination emphasizes multifaceted contributions to cellular function. UBE2D1 Protein, Human (GST) is the recombinant human-derived UBE2D1 protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2D1 Protein, Human (GST) is 147 a.a., with molecular weight of ~40.0 kDa.

UBE2D1 蛋白通过接受 E1 复合物中的泛素并促进其与多种蛋白质的共价连接，在细胞过程中发挥着关键作用（来源）。在体外，UBE2D1 表现出对“Lys-48”连接的多聚泛素化的催化偏好，展示了其在修饰靶蛋白方面的多功能性（来源）。UBE2D1 作为短寿命和异常蛋白选择性降解的关键介质，还参与 E6/E6-AP 诱导的 p53/TP53 泛素化。此外，它还促进 PEX5 的泛素化，并协调 STUB1、TRAF6 和 TRIM63/MURF1 的自动泛素化（来源）。UBE2D1 表现出在体外泛素化 STUB1 相关 HSP90AB1 的能力，强调了它参与复杂的细胞途径（来源）。值得注意的是，UBE2D1 对泛素的任何特定赖氨酸残基缺乏固有的特异性，突出了其在泛素修饰过程中的动态性质（来源）。它在 IRF3 病毒激活和 CYP3A4 多泛素化介导中的重要作用进一步强调了 UBE2D1 对细胞功能的多方面贡献（来源）。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P51668 (M1-M147)

7321  [NCBI]

MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM

Approximately 40.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerin, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.