UBE2K 蛋白, Human (GST)

UBE2K Protein, a vital component of the ubiquitin-proteasome system, functions as an E2 ubiquitin-conjugating enzyme, crucial for attaching ubiquitin to diverse substrates. In vitro, UBE2K catalyzes 'Lys-48'-linked polyubiquitin chains, particularly with or without the BRCA1-BARD1 E3 ligase complex. It participates in the selective degradation of short-lived proteins, including endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. UBE2K exhibits ubiquitination activity towards targets like huntingtin and p53/TP53, and potentially NF-kappa-B. Its role in viral infections involves contributing to MHC class I heavy chain degradation and participating in HPV E7 protein-dependent degradation of RB1. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2K Protein, Human (GST) is 200 a.a., with molecular weight of ~45.0 kDa.

UBE2K 是泛素-蛋白酶体系统的关键组成部分，作为 E2 泛素结合酶，在泛素与不同底物蛋白的共价连接中发挥着至关重要的作用。体外研究揭示其催化“Lys-48”连接的多聚泛素链合成的能力，特别是在存在或不存在 BRCA1-BARD1 E3 泛素-蛋白质连接酶复合物的情况下。值得注意的是，UBE2K 参与介导短寿命和异常蛋白的选择性降解，参与错误折叠的管腔蛋白的内质网相关降解 (ERAD) 等过程。此外，UBE2K 对各种靶标具有泛素化活性，包括亨廷顿蛋白、p53/TP53 和潜在的 NF-kappa-B。此外，它还参与病毒感染，其中 UBE2K 可能有助于 MHC I 类重链的降解，并参与 HPV E7 蛋白依赖性的 RB1 降解。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P61086 (M1-N200)

3093  [NCBI]

MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

Approximately 45.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.