UBE2T 蛋白, Human (His)

UBE2T Protein, an essential ubiquitination component, serves as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to proteins. This multifaceted enzyme catalyzes monoubiquitination, crucial in MMC-induced DNA repair processes. Its significance is highlighted by specific association with the Fanconi anemia complex, collaborating with FANCL for FANCD2 monoubiquitination in the DNA damage response pathway. Beyond DNA repair, UBE2T mediates monoubiquitination of FANCL and FANCI and potentially contributes to BRCA1 ubiquitination and degradation. In vitro, UBE2T promotes various polyubiquitination types, showcasing involvement in diverse ubiquitin-dependent processes. UBE2T Protein, Human (His) is the recombinant human-derived UBE2T protein, expressed by E. coli, with N-6*His labeled tag. The total length of UBE2T Protein, Human (His) is 197 a.a., with molecular weight of 25-30 kDa.

UBE2T 是泛素化机制的重要组成部分，作为 E2 泛素结合酶，接受 E1 复合物中的泛素并催化其与其他蛋白质的共价附着。这种多面酶具有催化单泛素化的能力，在丝裂霉素-C (MMC) 诱导的 DNA 修复过程中发挥着至关重要的作用。UBE2T 的重要性因其与范可尼贫血复合体的特定关联而得到强调，在范可尼贫血复合体中，它与 E3 泛素蛋白连接酶 FANCL 协同催化 FANCD2 的单泛素化，这是 DNA 损伤反应途径中不可或缺的一步。除了在 DNA 修复中的作用外，UBE2T 通过介导 FANCL 和 FANCI 的单泛素化并可能促进 BRCA1 的泛素化和降解而表现出多功能性。此外，体外研究揭示了 UBE2T 促进各种类型的多泛素化的能力，展示了其参与多种泛素依赖性过程。

Human

E. coli

N-6*His

Q9NPD8 (M1-V197)

29089  [NCBI]

MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

25-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.