uPAR 蛋白, Human (HEK293, His)

The uPAR protein is a receptor for urokinase plasminogen activator and actively localizes and promotes plasmin formation. It mediates proteolysis-independent signaling activated by U-PA and undergoes negative feedback regulation. uPAR Protein, Human (HEK293, His) is the recombinant human-derived uPAR protein, expressed by HEK293 , with C-6*His labeled tag. The total length of uPAR Protein, Human (HEK293, His) is 281 a.a., with molecular weight of ~52 kDa.

uPAR 蛋白作为尿激酶纤溶酶原激活剂的受体，积极参与纤溶酶形成的定位和促进。此外，它还充当 U-PA 诱导的不依赖于蛋白水解的信号转导激活效应的介质。受到 U-PA 的负反馈调节，uPAR 蛋白会裂解成无活性形式。通常作为单体存在，它与多种蛋白质相互作用，包括 MRC2、SRPX2（通过 UPAR/Ly6 结构域）和 FAP (seprase)，后者相互作用发生在侵袭伪足膜的细胞表面。此外，uPAR 蛋白与 SORL1 相互作用，特别是通过 N 末端胞外域，并且这种相互作用与 PLAUR 内化的减少有关。值得注意的是，由 PLAUR、PLAU（尿激酶型纤溶酶原激活剂）和 SERPINE1 组成的三元复合物的形成也涉及与 SORL1 的相互作用。

Human

HEK293

C-6*His

Q03405 (L23-R303)

5329  [NCBI]

LRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYR

Approximately 52 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 8% Trehaolse, 2% Dextran-70, 50 mM NaCl, 0.05% Tween80, pH 8.5 or PBS, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.