1. 重组蛋白
  2. Cytokines and Growth Factors
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-A
  6. VEGF165 蛋白, Rat (CHO)

VEGF165 蛋白, Rat (CHO)

目录号: HY-P7311
COA 产品使用指南

VEGF165 蛋白, Rat (CHO)是一个有效的血管生成调节剂,并能与信号蛋白受体neuropilin1 结合。

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规格 价格 是否有货 数量
10 μg ¥2500 In-stock
50 μg ¥8500 In-stock
100 μg   询价  

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描述

VEGF165 Protein, Rat (CHO) is a potent mediator of angiogenesis and binds the semaphorin receptor, neuropilin1.

研究背景

血管内皮生长因子(VEGF 或 VEGFA)是胎儿和成人血管生成和血管发生的有效介质。它是 PDGF 家族的一员,其特征是存在八个保守的半胱氨酸残基和一个胱氨酸结结构[1]。VEGF165 似乎是最丰富、最有效的异构体,其次是 VEGF121 和 VEGF189[1][2]。VEGF165 结合信号蛋白受体 Neuropilin1,并促进与 VEGF R2[3] 形成复合物。

生物活性

The ED50 is <4 ng/mL as measured by HUVEC cells, corresponding to a specific activity of >2.5 × 105 units/mg.

种属

Rat

表达系统

CHO

标签

Tag Free

蛋白编号

P16612-2 (A27-R190)

基因 ID

83785  [NCBI]

蛋白结构
N-term
VEGF165 (A27-R190)
Accession # P16612-2
C-term
中文名
重组大鼠血管内皮生长因子165 (CHO 表达)
同用名
rRtVEGF165; VEGF-AA; VPF
氨基酸序列

APTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

分子量

Approximately 35-48 kDa (Disulfide-linked homodimer)

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

VEGF165 Protein, Rat (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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