VHL 蛋白, Human (His)

VHL protein is an important component of the von Hippel-Lindau ubiquitination complex and plays a key role in ubiquitination and proteasomal degradation. As a target recruitment subunit in the E3 ubiquitin ligase complex, VHL specifically recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions and regulates cellular responses to oxygen levels. VHL Protein, Human (His) is the recombinant human-derived VHL protein, expressed by E. coli , with N-6*His labeled tag. The total length of VHL Protein, Human (His) is 213 a.a., with molecular weight of ~34 kDa.

VHL 蛋白在泛素化和随后通过 von Hippel-Lindau 泛素化复合物进行的蛋白酶体降解中发挥着关键作用。作为 E3 泛素连接酶复合物中的目标招募亚基，VHL 在常氧条件下特异性招募羟基化缺氧诱导因子 (HIF)。这种调节机制有助于调节细胞对氧水平变化的反应。此外，VHL 通过与 HIF1A、HIF1AN 和组蛋白脱乙酰酶相互作用参与转录抑制，进一步影响响应环境信号的基因表达。值得注意的是，VHL 表现出针对 ADRB2 的氧响应性泛素化活性。此外，它通过促进 RPTOR 的泛素化和降解，充当 mTORC1 的负调节因子。VHL 的多方面作用强调了其在细胞稳态、蛋白质修饰以及参与氧传感和反应的分子途径的协调中的重要性。

Human

E. coli

N-6*His

P40337 (M1-D213)

7428  [NCBI]

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD

Approximately 34 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.