Vimentin 蛋白, Human (sf9, His)

Vimentin, a class-III intermediate filament, predominantly exists in non-epithelial cells, particularly mesenchymal cells. This structural protein forms networks linking the nucleus, endoplasmic reticulum, and mitochondria. Teaming up with LARP6, vimentin stabilizes messenger RNAs (mRNAs) of type I collagen, notably CO1A1 and CO1A2, showcasing its role in regulating extracellular matrix components crucial for cellular structure and integrity. Vimentin Protein, Human (sf9, His) is the recombinant human-derived Vimentin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Vimentin Protein, Human (sf9, His) is 466 a.a., with molecular weight of ~55.11 kDa.

波形蛋白是一种 III 类中间丝，主要存在于非上皮细胞，特别是间充质细胞中。这种蛋白质形成一个结构网络，从侧面或末端连接到细胞核、内质网和线粒体。波形蛋白与 LARP6 合作，在稳定 I 型胶原蛋白的信使 RNA (mRNA)（特别是 CO1A1 和 CO1A2）方面发挥着至关重要的作用，强调其参与对细胞结构和完整性至关重要的细胞外基质成分的调节。

Human

Sf9 insect cells

C-His

P08670 (M1-E466)

7431  [NCBI]

MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Approximately 55.11 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 40% acetonitrile, 0.1% TFA. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.