1. 重组蛋白
  2. Viral Proteins
  3. Zika Virus Proteins
  4. Zika Virus E proteins
  5. Zika virus E/Envelope 蛋白 (Domain I, His-MBP)

Zika virus E/Envelope 蛋白 (Domain I, His-MBP)

目录号: HY-P73536
产品使用指南

Genome polyprotein 是共价连体的较小蛋白质链,是病毒生存和复制的关键组成部分。Zika virus E/Envelope 蛋白 (Domain I, His-MBP) 是重组的 寨卡病毒 E/包膜蛋白,由 E. coli 表达,带有 N-His, N-MBP 标签。Zika virus E/Envelope 蛋白 (Domain I, His-MBP) ,分子量约为 ~57.2 kDa。

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描述

Genome polyprotein is a smaller protein chain of covalent linkages that is a key component of virus survival and replication. Zika virus E/Envelope Protein (Domain I, His-MBP) is the recombinant Virus-derived Zika virus E/Envelope protein, expressed by E. coli , with N-His, N-MBP labeled tag. Zika virus E/Envelope Protein (Domain I, His-MBP), has molecular weight of ~57.2 kDa.

研究背景

Genome polyprotein 是一系列具有相似或不同功能的蛋白质单元,已被单细胞或多细胞生物体广泛利用,作为无数分子活动的浓缩器。Genome polyprotein 是共价连体的较小蛋白质链,在自然界中作为组织病毒(包括 HIV)蛋白质组的通用手段。Genome polyprotein 作为 NS4B 的信号肽,是 NS4B 抗干扰素活性所必需的。Genome polyprotein 通过干扰宿主 Dicer 抑制 RNA 沉默。Genome polyprotein 可能对病毒出芽起作用。通过 M 外结构域激活线粒体凋亡通路发挥细胞毒性作用。可能显示病毒蛋白活性[1][2]

种属

Virus

表达系统

E. coli

标签

N-His;N-MBP

蛋白编号

ALU33341 (I291-G399&E426-G485&S576-G592)

基因 ID

/

同用名
Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein (Domain I, His-MBP)
分子量

Approximately 57.2 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Zika virus E/Envelope Protein (Domain I, His-MBP) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Zika virus E/Envelope Protein (Domain I, His-MBP)
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HY-P73536
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