1. 诱导疾病模型产品 Cell Cycle/DNA Damage Apoptosis Autophagy
  2. 泌尿系统疾病模型 DNA Alkylator/Crosslinker Ferroptosis Autophagy
  3. Cisplatin

Cisplatin  (Synonyms: 顺铂; cis-Platinum; CDDP; cis-Diaminodichloroplatinum)

目录号: HY-17394 纯度: 99.84%
COA 产品使用指南

Cisplatin (CDDP) 是一种抗肿瘤的化疗剂,它与 DNA 交联引起癌细胞中 DNA 损伤。Cisplatin 可激活铁死亡 (ferroptosis) 并诱导自噬 (autophagy)。

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Cisplatin Chemical Structure

Cisplatin Chemical Structure

CAS No. : 15663-27-1

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 372 篇科研文献

WB
IF

    Cisplatin purchased from MCE. Usage Cited in: Gut Microbes. 2023 Jan-Dec;15(1):2197836.  [Abstract]

    Cisplatin (CDDP; 3.33 μM or 1.67 μM; 96 h) increases the protein expression of p53 and p21 in ECA-109 and KYSE-150 cells (3.33 μM 1 μg/ml in ECA-109 cells, 1.67 μM 0.5 μg/ml in KYSE-150 cells).

    Cisplatin purchased from MCE. Usage Cited in: Gut Microbes. 2023 Jan-Dec;15(1):2197836.  [Abstract]

    Cisplatin (CDDP; 3.33 μM or 1.67 μM; 96 h) suppresses the proliferation of ECA-109 and KYSE-150 cells (3.33 μM 1 μg/ml in ECA-109 cells, 1.67 μM 0.5 μg/ml in KYSE-150 cells).

    Cisplatin purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2023 May 20.

    Cisplatin (25, 50 μM; 24 h) significantly increases the expression of caspase-3 in PK-15 cells.

    Cisplatin purchased from MCE. Usage Cited in: Nat Commun. 2019 Aug 21;10(1):3761.  [Abstract]

    IB analysis of the level of cleaved-caspase 3 and cleaved-PARP1 in the indicated chemotherapy-treated xenograft tumors. GAPDH served as the loading control.

    Cisplatin purchased from MCE. Usage Cited in: Nat Commun. 2019 Aug 21;10(1):3761.  [Abstract]

    Genotoxic stress-induced β-catenin signaling is activated via a TCF- or FOXO-independent mechanism. Representative images of the subcellular localization of β-catenin in the indicated cells treated with CPT (10 μM, 1 h), IR (10 Gy), and CDDP (10 μM, 1 h), as analyzed by immunofluorescence staining.

    Cisplatin purchased from MCE. Usage Cited in: Cancer Res. 2018 Oct 1;78(19):5694-5705.  [Abstract]

    WT and S47 E1A/RAS cells are treated with 10 M Cisplatin (CDDP) for 24 hours. Cells are then fractionated into three fractions: whole cell lysate (W), mitochondria (M), and cytosol (C).

    Cisplatin purchased from MCE. Usage Cited in: Cancer Res. 2018 Oct 1;78(19):5694-5705.  [Abstract]

    WT and S47 E1A/RAS cells are treated with 10μM CDDP for 24 hours in the presence or absence of the protein synthesis inhibitor Cycloheximide (2.5 g/mL). Cell lysates are subjected to Western blot analysis, and immunoblotted for cleaved caspase-3, p53, p21, and HSP90 (loading control). CDDP: cisplatin

    Cisplatin purchased from MCE. Usage Cited in: Oncol Lett. 2018 May;15(5):6469-6474.  [Abstract]

    MDA-MB-231 cells are grown and treated with CC-5013 (Len), Cisplatin (Cis), or their combination for 72 h and then subjected to western blot analysis.

    Cisplatin purchased from MCE. Usage Cited in: Oncol Lett. 2018 Feb;15(2):2583-2589.  [Abstract]

    MLN4924 increases CDDP induced apoptosis of esophageal cancer cells. EC1 and Kyse450 cells are treated with 1.6 μg/mL CDDP alone or in combina¬tion with 0.3 μM MLN for 72 h. Cleaved PARP are detected by western blotting.

    Cisplatin purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Aug 24;682:112-117.  [Abstract]

    After treatment of CDDP with or without the autophagy inhibitor CQ, the expression of LC3-II in the CQ+CDDP group is less than that in the CQ group but is higher than that in the CDDP group at 24 h and 96 h.

    Cisplatin purchased from MCE. Usage Cited in: Cell Signal. 2017 May 1;36:108-116.  [Abstract]

    PAQR3 affects DNA damage repair. AGS cells are treated with different doses of VP-16 (for 24 h), Cisplatin (for 24 h) and NSC 123127 (for 10 h) as indicated, followed by immunoblotting with the antibodies.

    Cisplatin purchased from MCE. Usage Cited in: Oncotarget. 2017 Apr 25;8(17):29125-29137.  [Abstract]

    Knockdown of ROC1 significantly enhances CDDP-induced apoptosis. TE1 and Kyse450 cells are transfected with siROC1 for 48 h and then treated with 1 μg/mL CDDP for 48 h. Knockdown efficiency and cleaved PARP are assessed by western blotting analysis. Protein expression is quantified and statically analyzed.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Cisplatin (CDDP) is an antineoplastic chemotherapy agent by cross-linking with DNA and causing DNA damage in cancer cells. Cisplatin activates ferroptosis and induces autophagy[1][2][3].

    IC50 & Target

    DNA Alkylator/Crosslinker[1]

    体外研究
    (In Vitro)

    Cisplatin (CDDP) 以剂量依赖性方式引起 HeLa 细胞凋亡,浓度为 30 μM 的 Cisplatin 导致 24 小时处理后 90% 以上的细胞死亡。使用 30 μM 浓度检查 Cisplatin 诱导的细胞凋亡的动力学。Cisplatin 激活 MEK/ERK 信号通路,20 和 30 μM Cisplatin 均会导致显著的细胞凋亡,导致 ERK 的强烈激活[1]
    Cisplatin (50 μM) 在肾近端小管细胞 (RPTC) 中产生时间依赖性细胞凋亡,导致细胞收缩,半胱天冬酶 3 活性增加 50 倍,磷脂酰丝氨酸外化增加 4 倍,以及 5-和染色质浓缩和 DNA 亚倍体分别增加 15 倍[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    在携带黑色素瘤的小鼠中,Cisplatin (CDDP;4 mg/kg BW) 可减小实体瘤的大小和重量,添加 Cisplatin 的 HemoHIM 可促进肿瘤大小和重量的减小[3]
    与对照大鼠相比,Cisplatin 给药导致肾脏重量占总体重的百分比、尿量、血清肌酸酐和血尿素氮显著增加约 132%、315%、797% 和 556%[4]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    分子量

    300.05

    Formula

    Cl2H6N2Pt

    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    中文名称

    顺铂

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    In Vitro: 

    DMF 中的溶解度 : 14.17 mg/mL (47.23 mM; 超声加热助溶; DMSO can inactivate Cisplatin's activity)

    H2O 中的溶解度 : 1 mg/mL (3.33 mM; 超声助溶 (<60°C); DMSO can inactivate Cisplatin's activity)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.3328 mL 16.6639 mL 33.3278 mL
    5 mM 0.6666 mL 3.3328 mL 6.6656 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: PBS

      Solubility: 0.91 mg/mL (3.03 mM); 澄清溶液; 超声助溶 (<60°C)

    • 方案 二

      请依序添加每种溶剂: 50% PEG300    50% Saline

      Solubility: 10 mg/mL (33.33 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.84%

    参考文献
    Cell Assay
    [1]

    HeLa and A549 cells are maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 100 units of Penicillin, and 100 μg of Streptomycin/mL. They are cultured at 37°C in a humidified chamber containing 5% CO2. For the induction of apoptosis, cells are plated in 60-mm dishes 1 day prior to Cisplatin (0-30 μM) treatment[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3][4]

    Mice[3]
    Mice are divided randomly into three groups (Control, Cisplatin and Cisplatin+HemoHIM), and each group consists of twenty mice. B16F0 melanoma (5×105 cells/mouse) is inoculated into subcutaneous femoral left region of mice at 3 days before an initial injection of Cisplatin. Cisplatin is injected intraperitoneally at 4 mg/kg body weight (B.W.) on day 0, 7 and 14 (total three injections). Experimental group is intubated with HemoHIM at a final concentration of 100 mg/kgB.W. by everyday from day -1 to day 16, while the control group received only water. On day 17 after initial injection of Cisplatin, all mice of each group are experimented, respectively, to evaluate tumor weight or tumor size. The tumor size is calculated as follows: tumor size=ab2/2, where a and b are the larger and smaller diameters, respectively.
    Rats[4]
    Male Sprague-Dawley rats weighing 200 to 250 g are divided at random into 4 groups of 4 or 5 animals each. The first group (control) received a vehicle (5% carboxymethyl cellulose sodium solution (CMC-Na), 5 mL/kg body wt., p.o.) used for Capsaicin (Cap). The second group received Cap (10 mg/kg/d, p.o.) in 5% CMC-Na (5 mL/kg), and the third received 5% CMC-Na for 6 consecutive days injected with Cisplatin (5 mg/kg in physiological saline solution, i.p.). The fourth group received Cap (10 mg/kg/d, p.o.) in 5% CMC-Na for 6 consecutive days after Cisplatin injection (5 mg/kg, i.p.). For all groups, Cap or vehicle is given twice daily. The selected Cap concentration and the dose administration schedule without inducing any rat intestinal damage are chosen using data from our preliminary experiments.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMF 1 mM 3.3328 mL 16.6639 mL 33.3278 mL 83.3195 mL
    DMF 5 mM 0.6666 mL 3.3328 mL 6.6656 mL 16.6639 mL
    10 mM 0.3333 mL 1.6664 mL 3.3328 mL 8.3319 mL
    15 mM 0.2222 mL 1.1109 mL 2.2219 mL 5.5546 mL
    20 mM 0.1666 mL 0.8332 mL 1.6664 mL 4.1660 mL
    25 mM 0.1333 mL 0.6666 mL 1.3331 mL 3.3328 mL
    30 mM 0.1111 mL 0.5555 mL 1.1109 mL 2.7773 mL
    40 mM 0.0833 mL 0.4166 mL 0.8332 mL 2.0830 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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