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  3. Fluorescent Dye
  4. FHZ

FHZ 是一个荧光探针。

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FHZ Chemical Structure

FHZ Chemical Structure

CAS No. : 1883737-63-0

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规格 价格 是否有货
5 mg ￥2200
询问价格 & 货期
10 mg ￥3700
询问价格 & 货期

* Please select Quantity before adding items.

FHZ 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

FHZ is a fluorescent probe.

体外研究
(In Vitro)

After loaded with probe FHZ and treated with HClO and H2O2/EDTA-Fe2+ in order, HeLa cells display the bright fluorescences from both cyan and green channels. FHZ can give out two different fluorescent signals in the presence of both ?OH and HClO, suggesting the synchronous discrimination of ?OH and HClO by a dual-channel detection model with two exciting wavelengths. Probe FHZ shows very high specificity to the detections of ?OH and HClO with the excitations at 410 and 490 nm, respectively. The probe FHZ can efficiently enter the cellular mitochondria and exhibit the differentiable/visual capabilities to the endogenous ?OH and HClO by the dual fluorescent responses[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

FHZ 相关抗体:
体内研究
(In Vivo)

Probe FHZ can rapidly be absorbed into the blood circulation system from the zebrafish intestine, and spread out whole zebrafish tissues, and keep its stability in the blood, organs and tissues in the absence of ROS. The probe can keep its stability in biological environments and only selectively react with ?OH and HClO species[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

478.49

Formula

C26H26N2O7
CAS 号
性状

固体

颜色

Light brown to brown

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (208.99 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0899 mL 10.4495 mL 20.8991 mL
5 mM 0.4180 mL 2.0899 mL 4.1798 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献
Cell Assay
[1]

HeLa cells and RAW264.7 macrophages are cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplied with 10% fetal bovine serum (FBS) and 1% antibotics (penicillin and streptomycin) at 37°C in humidified incubator containing 5% CO2. The cells are seeded into glass-bottomed dishes and cultured for 24 h. Subsequently, the cells are incubated with FHZ for 30 min at 37°C and then washed with PBS buffer three times. Each treatment of cells with H2O2, EDTA-Fe2+, HClO or scavengers kept 30 min[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Wild type zebrafish is used in this study. Seven-day old fertilized zebrafish embryos are cultured in 50 μM FHZ for 30 min, and then the zebrafish embryos are transferred to fresh water. A FHZ-loaded zebrafish is fixed under confocal microscope using 2% agarose gel to keep its living state for fluorescent imaging. In order to observe the release of •OH in fresh wound, the ventral fin of the FHZ-loaded zebrafish is carefully cut a small wound using a blade. After raised for 20 min in water, the wound of injured zebrafish is imaged using confocal microscope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

FHZ 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0899 mL 10.4495 mL 20.8991 mL 52.2477 mL
5 mM 0.4180 mL 2.0899 mL 4.1798 mL 10.4495 mL
10 mM 0.2090 mL 1.0450 mL 2.0899 mL 5.2248 mL
15 mM 0.1393 mL 0.6966 mL 1.3933 mL 3.4832 mL
20 mM 0.1045 mL 0.5225 mL 1.0450 mL 2.6124 mL
25 mM 0.0836 mL 0.4180 mL 0.8360 mL 2.0899 mL
30 mM 0.0697 mL 0.3483 mL 0.6966 mL 1.7416 mL
40 mM 0.0522 mL 0.2612 mL 0.5225 mL 1.3062 mL
50 mM 0.0418 mL 0.2090 mL 0.4180 mL 1.0450 mL
60 mM 0.0348 mL 0.1742 mL 0.3483 mL 0.8708 mL
80 mM 0.0261 mL 0.1306 mL 0.2612 mL 0.6531 mL
100 mM 0.0209 mL 0.1045 mL 0.2090 mL 0.5225 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FHZ1883737-63-0Fluorescent DyeInhibitorinhibitorinhibit

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