1. Cell Cycle/DNA Damage Anti-infection Apoptosis
  2. DNA/RNA Synthesis HSV Apoptosis Antibiotic Orthopoxvirus
  3. Aphidicolin

Aphidicolin  (Synonyms: 阿非迪霉素)

目录号: HY-N6733 纯度: ≥99.0%
COA 产品使用指南

Aphidicolin 是 DNA 聚合酶 α 和 δ 抑制剂,通过干扰 DNA 聚合酶的活性来阻止细胞有丝分裂。Aphidicolin 是一种来源于 Cephalosporium aphidicola 的抗生素, 能够抑制细胞脱氧核糖核酸合成和单纯疱疹病毒生长。Aphidicolin 具有抗正痘病毒活性,能够增强阿拉伯糖基核苷对细胞凋亡 (apoptosis) 的诱导作用。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Aphidicolin Chemical Structure

Aphidicolin Chemical Structure

CAS No. : 38966-21-1

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
1 mg ¥1500
In-stock
5 mg   询价  
10 mg   询价  

* Please select Quantity before adding items.

Customer Review

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Aphidicolin is an inhibitor of DNA polymerase α and δ, prevents mitotic cell division by interfering DNA polymerase activity. Aphidicolin is an antibiotic produced by mold Cephalosporium aphidicola, inhibits cellular deoxyribonucleic acid synthesis and the growth of herpes simplex virus. Aphidicolin exhibits anti-orthopoxvirus activity and potentiates apoptosis induced by arabinosyl nucleosides in a human promyelocytic leukemia cell line[1][2][3].

体外研究
(In Vitro)

Aphidicolin (0.5 μM, 5 μM; 0-5 d) selectively kills neuroblastoma cells, but shows moderate cytotoxicity on normal human embryonal cells and HeLa, H9, A549 and Caco-2 cell lines[4].
Aphidicolin (0.4 μg/mL; 3 d) arrests cell cycle at G2 phase[5].
Aphidicolin (100 nM-10 μM; 48 h) inhibits cell proliferation via the p53-GADD45β pathway and (1 μM; 24 h) induces apoptosis in AtT-20 cells[6].
Aphidicolin (10 μM; 0-6 h) decreases the phosphorylation of Akt, (100 nM-10 μM; 24 h) increases the mRNA levels of the stress response gene growth arrest and DNA damage-inducible 45β (GADD45β), a putative downstream target of p53[6].
Aphidicolin (10 μM; 0-6 h) inhibits Varicella-zoster virus (VZV) with EC50s of 0.5-0.6 μM, with low cytotoxicity[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[4]

Cell Line: NB cell lines: UKF-NB-1/2/3 and IMR-32
Concentration: 0.5 μM, 5μM
Incubation Time: 1, 2, 3, 4, 5 days
Result: Resulted in cellular enlargement and extension of cellular processes before cell lysis occurred.

Cell Cycle Analysis[5]

Cell Line: Normal human diploid cells
Concentration: 0.4 μg/mL
Incubation Time: 3 days or 7 days
Result: Resulted more than 80% of the cells moved through S phase and were accumulated at G2 phase.
Inbihited the growth of the cells completely without causing apparent cell death.

Western Blot Analysis[6]

Cell Line: AtT-20 cells pituitary corticotroph tumor cells
Concentration: 10 µM
Incubation Time: 0 min, 5 min, 30 min, 2 h, 6 h, 24 h
Result: Inhibited Akt phosphorylation in AtT-20 cells during 5 min-2 h, in a time-dependent manner.
Increased protein level of p27 during 30 min-6 h, and remarkably increased p53 level at 24 h.
体内研究
(In Vivo)

Aphidicolin, has been developed with higher solubility agent, namely Aphidicolin glycinate (AG; NSC 303812).
Aphidicolin glycinate (100 mg/kg; i.p.; once every 3 h; 9 d) shows anti-tumor activity against the implanted B16 melanoma and M5076 sarcoma in murine, producing maximum increased life spans of 75%[8].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: M5076 sarcoma s.c. model or B16 melanoma i.p. model in murine[8]
Dosage: 60 mg/kg, 100 mg/kg, 300 mg/kg
Administration: Intraperitoneal injection; once every 3 h; 9 days
Result: Inhibited tumor growth significantly.
分子量

338.48

Formula

C20H34O4

CAS 号
性状

固体

颜色

White to off-white

中文名称

阿非迪霉素

结构分类
初始来源

Cephalosporium aphidicola

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 50 mg/mL (147.72 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9544 mL 14.7719 mL 29.5438 mL
5 mM 0.5909 mL 2.9544 mL 5.9088 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥99.0%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9544 mL 14.7719 mL 29.5438 mL 73.8596 mL
5 mM 0.5909 mL 2.9544 mL 5.9088 mL 14.7719 mL
10 mM 0.2954 mL 1.4772 mL 2.9544 mL 7.3860 mL
15 mM 0.1970 mL 0.9848 mL 1.9696 mL 4.9240 mL
20 mM 0.1477 mL 0.7386 mL 1.4772 mL 3.6930 mL
25 mM 0.1182 mL 0.5909 mL 1.1818 mL 2.9544 mL
30 mM 0.0985 mL 0.4924 mL 0.9848 mL 2.4620 mL
40 mM 0.0739 mL 0.3693 mL 0.7386 mL 1.8465 mL
50 mM 0.0591 mL 0.2954 mL 0.5909 mL 1.4772 mL
60 mM 0.0492 mL 0.2462 mL 0.4924 mL 1.2310 mL
80 mM 0.0369 mL 0.1846 mL 0.3693 mL 0.9232 mL
100 mM 0.0295 mL 0.1477 mL 0.2954 mL 0.7386 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Aphidicolin
目录号:
HY-N6733
需求量: