2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. ATP disodium salt hydrate

ATP disodium salt hydrate  (Synonyms: Adenosine 5'-triphosphate disodium salt hydrate)

目录号: HY-W010735 纯度: 99.64%
COA 产品使用指南 技术支持

ATP disodium salt hydrate (Adenosine 5'-triphosphate disodium salt hydrate) 是体内能量储存和代谢的重要物质，为代谢提供能量，同时在细胞中作为辅酶发挥作用。ATP disodium salt hydrate 是免疫和炎症中重要的内源性信号分子。

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ATP disodium salt hydrate

ATP disodium salt hydrate Chemical Structure

CAS No. : 34369-07-8

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500 mg ￥350
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1 g ￥450
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5 g ￥900
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Customer Review

Other Forms of ATP disodium salt hydrate:

ATP disodium salt hydrate 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 109 篇科研文献

WB
Cell Viability Assay
RT-PCR

    ATP disodium salt hydrate purchased from MCE. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1 (one experiment, n = 3). Supernatant (Sup) IL-1β (P17) and cellular lysates (Lys) pro-IL-1β; (I) supernatant caspase-1 (P20) and lysates pro-caspase-1.

    ATP disodium salt hydrate purchased from MCE. Usage Cited in: Transl Res. 2023 May:255:1-13.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP disodium salt hydrate purchased from MCE. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP disodium salt hydrate purchased from MCE. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP disodium salt hydrate purchased from MCE. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs and BMMs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP or 100 ng/mL for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP disodium salt hydrate purchased from MCE. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    ATP disodium salt hydrate (Adenosine 5'-triphosphatedisodium salt hydrate) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt hydrate is an important endogenous signaling molecule in immunity and inflammation[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    体外研究
    (In Vitro)

    ATP disodium salt hydrate（5mM；1 小时）与 LPS（1 μg/mL）联合处理对 HGF 中 NLRP3 炎症小体的活化具有协同作用[3]。
    ATP disodium salt hydrate（2 mM；0.5-24 小时）以 caspase-1 活化依赖性方式诱导体外骨髓来源的巨噬细胞 (BMDM) 分泌白细胞介素 1β、KC 和 MIP-2[4]。
    ATP disodium salt hydrate 刺激细胞因子和趋化因子的分泌，炎症小体的活化直接和间接地诱导体外中性粒细胞趋化性[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP disodium salt hydrate 相关抗体:
    体内研究
    (In Vivo)

    ATP disodium salt hydrate（50 mg/kg；腹腔注射）可保护小鼠免受体内细菌感染[4]。
    ATP disodium salt hydrate 可诱导 IL1β、KC 和 MIP-2 的分泌以及中性粒细胞的体内募集[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, before bacterial (E. coli) challenge
    Result: Protected mice from bacterial infection.
    Clinical Trial
    Formula

    C10H14N5O13Na2P3.xH2O
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    腺苷-5'-三磷酸二钠盐水合物
    结构分类
    初始来源

    widespread
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 100 mg/mL (超声助溶)

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL; 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.64%

    COA (290 KB) HNMR (243 KB) RP-HPLC (269 KB)

    产品使用指南 (1538 KB)
    参考文献

    ATP disodium salt hydrate 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP disodium salt34369-07-8Adenosine 5'-triphosphate disodium salt腺苷-5'-三磷酸二钠盐水合物Endogenous MetaboliteenergystoragemetabolismimmunityinflammationNLRP3inflammasomecytokineInhibitorinhibitorinhibit

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    目录号：
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