1. Epigenetics TGF-beta/Smad Anti-infection
  2. PKC HIV Bacterial
  3. Bryostatin 1

Bryostatin 1  (Synonyms: 苔藓抑素1)

目录号: HY-105231 纯度: ≥99.0%
COA 产品使用指南

Bryostatin 1 是一种天然的大环内酯类化合物,从苔藓虫 Bugula neritina 中分离出来,也是一种有效的中枢神经系统 (CNS) 渗透性的 PKC 调节剂。Bryostatin 1 以 Ki 值为 8.07 nM 和 0.45 nM 分别结合到分离的 Munc13-1 的 C1 域和全长的 Munc13-1 蛋白。Bryostatin 1 具有抗癌,抗炎,神经保护,抗 HIV-1 感染的特性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Bryostatin 1 Chemical Structure

Bryostatin 1 Chemical Structure

CAS No. : 83314-01-6

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥5200
In-stock

* Please select Quantity before adding items.

Customer Review

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Bryostatin 1 is a natural macrolide isolated from the bryozoan Bugula neritina and is a potent and central nervous system (CNS)-permeable PKC modulator. Bryostatin 1 binds to the isolated C1 domain of Munc13-1 and the full-length Munc13-1 protein with Kis of 8.07 nM and 0.45 nM, respectively. Bryostatin 1 has anti-cancer, anti-inflammatory, neuroprotective, anti-HIV-1 infection properties[1][2][3][4].

IC50 & Target

HIV-1

 

体外研究
(In Vitro)

Bryostatin 1 (1 µM; 5 minutes; HT22 cells) treatment successfully recruits Munc13-1 from the cytosol to the plasma membrane. Effects of Bryostatin 1 on the other Munc13 family members, ubMunc13-2 and bMunc13-2, resembled those of Munc13-1 for translocation [1].
The increased level of expression of Munc13-1 following a 24 h incubation with Bryostatin 1 in both HT22 and primary mouse hippocampal cells is observed[1].
Bryostatin 1 can also affect the immune system by modulating dendritic cells (DCs) via toll-like receptor 4 (TLR4) through the MyD88-independent pathway, which favors an anti-inflammatory environment by inducing a type 2 phenotype that promotes the differentiation of CD4+ T-helper (Th) lymphocytes into Th2 versus Th1 effector cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HT22 cells
Concentration: 1 µM
Incubation Time: 5 minutes
Result: Caused Munc13-1 to transfer to the membrane fraction.
体内研究
(In Vivo)

Bryostatin 1 (30 μg/kg; intraperitoneal injection; 3 d per week; for 2 weeks; C57BL/6J mice) treatment abolishes the onset of EAE[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6J mice (8-12-week-old) with MOG35-55[2]
Dosage: 30 μg/kg
Administration: Intraperitoneal injection; 3 d per week; for 2 weeks
Result: Abolished the onset of experimental autoimmune encephalomyelitis (EAE).
Clinical Trial
分子量

905.03

Formula

C47H68O17

CAS 号
性状

固体

颜色

White to off-white

中文名称

苔藓抑素1

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 50 mg/mL (55.25 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1049 mL 5.5247 mL 11.0494 mL
5 mM 0.2210 mL 1.1049 mL 2.2099 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥99.0%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1049 mL 5.5247 mL 11.0494 mL 27.6234 mL
5 mM 0.2210 mL 1.1049 mL 2.2099 mL 5.5247 mL
10 mM 0.1105 mL 0.5525 mL 1.1049 mL 2.7623 mL
15 mM 0.0737 mL 0.3683 mL 0.7366 mL 1.8416 mL
20 mM 0.0552 mL 0.2762 mL 0.5525 mL 1.3812 mL
25 mM 0.0442 mL 0.2210 mL 0.4420 mL 1.1049 mL
30 mM 0.0368 mL 0.1842 mL 0.3683 mL 0.9208 mL
40 mM 0.0276 mL 0.1381 mL 0.2762 mL 0.6906 mL
50 mM 0.0221 mL 0.1105 mL 0.2210 mL 0.5525 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Bryostatin 1
目录号:
HY-105231
需求量: