2. MCE Kits
  3. Cell Biology
  4. Cell Culture
  5. Sterile Antibiotic Solution
  6. Hygromycin B, Sterile

Hygromycin B, Sterile 

潮霉素 B 无菌溶液

Cat. No.: HY-K1051
Manual SDS

MCE Hygromycin B, Sterile 是一种氨基糖苷类抗生素，主要通过干扰 70S 核糖体易位和诱导对 mRNA 模板的错读而抑制细胞蛋白质的正常合成，从而导致细菌、真菌和哺乳动物细胞死亡。

Hygromycin B, Sterile

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (1 mL)   Apply now  
10 mL ￥880 In-stock
50 mL ￥3080 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Hygromycin B is an aminoglycosidic antibiotic purified from Streptomyces hygroscopicus. It acts by binding to the 70S subunit of the bacterial ribosome and inhibiting protein synthesis, leading to the death of bacteria, fungi and mammalian cells.

Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). Therefore, Hygromycin B can be used to select and maintain prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene. Low purity hygromycin can cause different toxic effects, so it is crucial to grasp its purity in the experiment.

Storage

Store at 4°C for 2 years.

Protocol

1.   The working concentration of Hygromycin B varies with cell type, media, growth conditions and cell metabolic rate.

2.   Before stable transfected cell lines can be selected, the optimal Hygromycin B concentration needs to be determined by performing a kill curve titration.

  1)   Seed the parental cell line in a suitable culture plate at a cell density of 20-25% and incubate for 24 hours at 37°C.

  2)   Remove medium and then add medium with various concentrations of Hygromycin B (such as 50, 100, 250, 500, 750, and 1000 μg/mL) and incubate at 37°C.

  3)   Refresh the selective medium every 2-3 days and observe the percentage of surviving cells over time.

  4)   Determine the lowest concentration of antibiotic that kills a large majority of the cells within 7-10 days. This concentration should be used for selection of a stable transfected cell line.
Components
Components HY-K1051-10 mL HY-K1051-50 mL
Hygromycin B, Sterile (50 mg/mL) 10 mL 50 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
潮霉素 B 无菌溶液
目录号：
HY-K1051
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