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  3. Magnesium sulfate, for cell culture

Magnesium sulfate, for cell culture  (Synonyms: 硫酸镁, 用于细胞培养)

目录号: HY-Y1267D 纯度: ≥99.0%
COA 产品使用指南 技术支持

Magnesium sulfate, for cell culture 适用于细胞培养。Magnesium sulfate 是一种钙拮抗剂和有效的 L 型钙通道抑制剂,也是一种宫缩抑制剂。Magnesium sulfate 具有抗炎、抗惊厥、血管舒张和神经保护的作用。Magnesium sulfate 可用于先兆子痫/子痫等疾病的研究。

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Magnesium sulfate, for cell culture

Magnesium sulfate, for cell culture Chemical Structure

CAS No. : 7487-88-9

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Magnesium sulfate, for cell culture is a bioreagent. Magnesium sulfate is a calcium antagonist and a potent L-type calcium channel inhibitor, as well as a tocolytic. Magnesium sulfate has anti-inflammatory, anticonvulsant, vasodilatory, and neuroprotective effects. Magnesium sulfate can be used in the research of diseases such as preeclampsia/eclampsia[1][2][3][4][5][6].

体外研究
(In Vitro)

Magnesium sulfate (3 mM) for cell culture 可抑制培养的子宫肌层细胞中磷脂酰肌醇-4,5-二磷酸特异性磷脂酶 C 活性和随后的钙释放[2]
Magnesium sulfate (20 mM; 6 小时) for cell culture 可抑制 RAW264.7 细胞中 LPS (HY-D1056) 诱导的炎症分子的产生和 NF-κB 的活化[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR[3]

Cell Line: RAW264.7 cells treated with 100 ng/mL of LPS (HY-D1056)
Concentration: 20 mM
Incubation Time: 6 h
Result: Inhibtied the levels of iNOS and COX-2.
体内研究
(In Vivo)

Magnesium sulfate (腹腔注射; 270 mg/kg, 随后每 20 分钟注射 27 mg/kg, 持续 4 小时, 最后再注射 270 mg/kg) for cell culture 可减轻 LPS (HY-D1056) 引起的胎鼠炎症相关脑损伤[4]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CD-1 out-bred timed pregnant mice, and intrauterine inflammation was induced by intrauterine injection of LPS (HY-D1056) on embryonic day 15[4]
Dosage: 270 mg/kg, followed by 27 mg/kg every 20 minutes for 4 hours, and then an additional 270 mg/kg
Administration: Intraperitoneal injection
Result: Prevented LPS-induced neuronal injury in fetal mouse brains, as evidenced by maintaining normal neuronal morphology, including dendritic processes and MAP2 staining, without significantly affecting the expression of proinflammatory cytokines or cell death markers.
分子量

120.37

Formula

MgSO4

CAS 号
性状

固体

颜色

White to off-white

中文名称

硫酸镁, 用于细胞培养

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

H2O 中的溶解度 : ≥ 100 mg/mL (830.77 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 8.3077 mL 41.5386 mL 83.0772 mL
5 mM 1.6615 mL 8.3077 mL 16.6154 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: ≥99.0%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 8.3077 mL 41.5386 mL 83.0772 mL 207.6929 mL
5 mM 1.6615 mL 8.3077 mL 16.6154 mL 41.5386 mL
10 mM 0.8308 mL 4.1539 mL 8.3077 mL 20.7693 mL
15 mM 0.5538 mL 2.7692 mL 5.5385 mL 13.8462 mL
20 mM 0.4154 mL 2.0769 mL 4.1539 mL 10.3846 mL
25 mM 0.3323 mL 1.6615 mL 3.3231 mL 8.3077 mL
30 mM 0.2769 mL 1.3846 mL 2.7692 mL 6.9231 mL
40 mM 0.2077 mL 1.0385 mL 2.0769 mL 5.1923 mL
50 mM 0.1662 mL 0.8308 mL 1.6615 mL 4.1539 mL
60 mM 0.1385 mL 0.6923 mL 1.3846 mL 3.4615 mL
80 mM 0.1038 mL 0.5192 mL 1.0385 mL 2.5962 mL
100 mM 0.0831 mL 0.4154 mL 0.8308 mL 2.0769 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Magnesium sulfate, for cell culture
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