1. Academic Validation
  2. Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus

Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus

  • Vet Microbiol. 2022 Sep;272:109511. doi: 10.1016/j.vetmic.2022.109511.
Rui-Cong Sun 1 Jia-Huan Hu 1 Xiao-Han Li 1 Chun-Chun Liu 1 Ya-Yun Liu 1 Jing Chen 1 Yi-Chen Yang 1 Bin Zhou 2
Affiliations

Affiliations

  • 1 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • 2 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China; Key Laboratory of Animal Bacteriology, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, China. Electronic address: zhoubin@njau.edu.cn.
Abstract

Classical swine fever virus (CSFV), a member of the Flaviviridae enveloped RNA virus family, results in an epidemic disease that brings serious economic losses to the pig industry worldwide. Valosin-containing protein (VCP/p97), a multifunctional active protein in cells, is related to the life activities of many viruses. However, the role of VCP in CSFV Infection remains unknown. In this study, it was first found that treatment of VCP inhibitors impaired CSFV propagation. Furthermore, overexpression or knockdown of VCP showed that it was essential for CSFV Infection. Moreover, confocal microscopy and immunoprecipitation assay showed that VCP was recruited for intracellular transport from early endosomes to lysosomes. Importantly, knockdown of VCP prevented CSFV to release from early endosomes, suggesting that VCP is a key factor for CSFV trafficking. Taken together, our findings first demonstrate that the endocytosis of CSFV into PK-15 cells requires the participation of VCP, providing the alternative approach for the discovery of novel anti-flaviviridae drugs.

Keywords

Classical swine fever virus; Early endosomes; Endocytosis; Lysosomes; Valosin-containing protein (VCP/p97).

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