1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) ADAMs/ADAMTSs
  4. ADAM 9
  5. ADAM9 蛋白, Human (HEK293, His)

ADAM9 蛋白, Human (HEK293, His)

目录号: HY-P77867
产品使用指南

ADAM9 是一种金属蛋白酶,可裂解并释放 TEK、KDR、EPHB4、CD40、VCAM1 和 CDH5,在肿瘤发生和血管生成中发挥关键作用。它通过整合素相互作用调节细胞运动,介导细胞-细胞和细胞-基质相互作用,并可能充当 APP 的 α 分泌酶,表明它参与神经生物学和神经退行性疾病。ADAM9 蛋白, Human (HEK293, His) 是重组的 ADAM9 蛋白,由 HEK293 表达,带有 C-His 标签。ADAM9 蛋白, Human (HEK293, His) 全长 669 个氨基酸,分子量为 40-45 kDa。

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描述

ADAM9, a metalloprotease, cleaves and releases TEK, KDR, EPHB4, CD40, VCAM1, and CDH5, playing a pivotal role in tumorigenesis and angiogenesis. It regulates cell motility through integrin interactions, mediates cell-cell and cell-matrix interactions, and may act as an alpha-secretase for APP, suggesting involvement in neurobiology and neurodegenerative disorders. ADAM9 Protein, Human (HEK293, His) is the recombinant human-derived ADAM9 protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM9 Protein, Human (HEK293, His) is 669 a.a., with molecular weight of 40-45 kDa.

研究背景

ADAM9 是一种金属蛋白酶,通过裂解和释放各种分子(包括 TEK、KDR、EPHB4、CD40、VCAM1 和 CDH5),在肿瘤发生和血管生成中发挥关键作用。其多方面的功能延伸到介导细胞-细胞和细胞-基质相互作用,从而通过与整合素的相互作用来调节细胞运动。此外,ADAM9 可能充当淀粉样前体蛋白 (APP) 的 α 分泌酶,突出显示其参与与神经生物学和神经退行性疾病相关的过程。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q13443 (A29-D697)

基因 ID
蛋白结构
N-term
ADAM9 (A29-D697)
Accession # Q13443
His
C-term
同用名
ADAM 9; Meltrin-gamma; Mdc9; Mltng; Adam9; Kiaa0021; AU020942; MDC9; mKIAA0021; CORD9; MCMP; MLTNG
分子量

40-45 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ADAM9 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ADAM9 Protein, Human (HEK293, His)
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