Animal-Free TNF beta 蛋白, Mouse (His)

The animal-free TNF beta protein acts as a homotrimeric cytokine that binds TNFRSF1A/TNFR1, TNFRSF1B/TNFBR, and TNFRSF14/HVEM. Animal-Free TNF beta Protein, Mouse (His) is the recombinant mouse-derived animal-FreeTNF beta protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free TNF beta Protein, Mouse (His) is 169 a.a., with molecular weight of ~19.36 kDa.

同源三聚体形式的 TNF β 蛋白通过结合 TNFRSF1A/TNFR1、TNFRSF1B/TNFBR 和 TNFRSF14/HVEM 发挥作用，其相互作用显示出高度的相似性。它以与 LTB 形成的异三聚体形式与 TNFRSF3/LTBR 结合，揭示了其在受体相互作用中的多功能性。淋巴毒素是由淋巴细胞产生的 TNF β 的异三聚体形式，在体外和体内均表现出针对多种肿瘤细胞的细胞毒性。它以同源三聚体和异源三聚体复合物的形式存在，后者由两个 LTB 和一个 LTA 亚基组成，或者在较小程度上由两个 LTA 和一个 LTB 亚基组成。值得注意的是，TNF beta 与 TNFRSF14 相互作用，进一步促进其多样化的免疫调节功能。

The ED₅₀ is <30 ng/mL as measure by its ability to induce cytotoxicity in L929 cells in the presence of vactinomycin D.

Mouse

E. coli

N-His

P09225 (L34-L202)

16992  [NCBI]

LSGVRFSAARTAHPLPQKHLTHGILKPAAHLVGYPSKQNSLLWRASTDRAFLRHGFSLSNNSLLIPTSGLYFVYSQVVFSGESCSPRAIPTPIYLAHEVQLFSSQYPFHVPLLSAQKSVYPGLQGPWVRSMYQGAVFLLSKGDQLSTHTDGISHLHFSPSSVFFGAFAL

Approximately 19.36 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.