2. 重组蛋白
  3. Enzymes & Regulators
  4. Lyases (EC 4) Carbonic Anhydrase
  5. Carbonic Anhydrase 14 (CA-XIV)
  6. Carbonic Anhydrase 14 蛋白, Mouse (275a.a, HEK293, His)

Carbonic Anhydrase 14 蛋白, Mouse (275a.a, HEK293, His)

目录号: HY-P70810
COA 产品使用指南

碳酸酐酶 14 (CA14) 蛋白催化二氧化碳的可逆水合，将其转化为碳酸氢根离子和质子。其酶活性对于生理过程、调节 pH 值和维持酸碱平衡至关重要。Carbonic Anhydrase 14 蛋白, Mouse (275a.a, HEK293, His) 是重组的 碳酸酐酶 14 蛋白，由 HEK293 表达，带有 C-6*His 标签。Carbonic Anhydrase 14 蛋白, Mouse (275a.a, HEK293, His) 全长 275 个氨基酸，分子量为 41-50 kDa。

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规格 价格 是否有货 数量
10 μg ￥1600 In-stock
50 μg ￥4500 In-stock
100 μg   询价  

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描述

The carbonic anhydrase 14 (CA14) protein catalyzes the reversible hydration of carbon dioxide, converting it into bicarbonate ions and protons. Its enzymatic activity is essential for physiological processes, regulating pH and maintaining acid-base balance. Carbonic Anhydrase 14 Protein, Mouse (275a.a, HEK293, His) is the recombinant mouse-derived Carbonic Anhydrase 14 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Carbonic Anhydrase 14 Protein, Mouse (275a.a, HEK293, His) is 275 a.a., with molecular weight of 41-50 kDa.
研究背景

碳酸酐酶 14 (CA14) 蛋白负责二氧化碳的可逆水合，在催化二氧化碳转化为碳酸氢根离子和质子方面发挥着关键作用。这种酶活性在各种生理过程中至关重要，有助于调节 pH 值和维持酸碱平衡。作为碳酸酐酶家族的一员，CA14 参与组织中二氧化碳转运和缓冲的关键生化反应，强调了其在细胞稳态中的重要性。
种属

Mouse
表达系统

HEK293
标签

C-6*His
蛋白编号

Q9WVT6 (A16-M290)
基因 ID

23831  [NCBI]
蛋白结构
N-term
Ca14 (A16-M290)
Accession # Q9WVT6
6*His
C-term
同用名
Carbonic Anhydrase 14; Carbonate Dehydratase XIV; Carbonic Anhydrase XIV; CA-XIV; CA14;
氨基酸序列

ADGGHHWTYEGPHGQDHWPTSYPECGGDAQSPINIQTDSVIFDPDLPAVQPHGYDQLGTEPLDLHNNGHTVQLSLPPTLHLGGLPRKYTAAQLHLHWGQRGSLEGSEHQINSEATAAELHVVHYDSQSYSSLSEAAQKPQGLAVLGILIEVGETENPAYDHILSRLHEIRYKDQKTSVPPFSVRELFPQQLEQFFRYNGSLTTPPCYQSVLWTVFNRRAQISMGQLEKLQETLSSTEEDPSEPLVQNYRVPQPLNQRTIFASFIQAGPLYTTGEM

分子量

41-50 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (210 KB)

产品使用指南 (1213 KB)

Carbonic Anhydrase 14 Protein, Mouse (275a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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