1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 4 (CA IV)
  5. Carbonic Anhydrase 4 蛋白, Human (His)

Carbonic Anhydrase 4 蛋白, Human (His)

目录号: HY-P7720
COA 产品使用指南

Carbonic Anhydrase 4 蛋白, Human (His)是一种在 E. coli 表达的,N 末端有 His 标签的重组人 Mouse Carbonic Anhydrase 4。Carbonic Anhydrase 4 (CA4) 蛋白是一种锌金属酶,催化 CO2 和 HCO3- 的可逆水合和脱水。

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规格 价格 是否有货 数量
10 μg ¥1654 In-stock
50 μg ¥4962 In-stock
100 μg   询价  

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描述

Carbonic Anhydrase 4 Protein, Human (His) a recombinant Mouse Carbonic Anhydrase 4 produced in E. coli, has a His tag at the N-terminus. The Carbonic Anhydrase 4 (CA4) protein is a zinc metalloenzyme that catalyzes the reversible hydration and dehydration of CO2 and HCO3-[1].

研究背景

人类碳酸酐酶 IV (CA4) 基因位于染色体 17q22 上,是 16 成员碳酸酐酶 (CA) 基因家族中第一个被鉴定的膜结合同工酶,包含 1,170 个碱基对。CA4酶参与胃酸的形成并参与酸碱稳态。CA4在正常人胃组织中表达。CA4可能通过调节细胞周期相关蛋白的表达来抑制细胞增殖,从而在胃癌(GC)肿瘤发生中发挥重要作用。CA4 可作为 GC 的诊断生物标志物和潜在的治疗靶点[1]

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

P22748 (A19-K283)

基因 ID

762  [NCBI]

蛋白结构
N-term
CA4 (A19-K283)
Accession # P22748
6*His
C-term
中文名
重组人碳酸酐酶 4,His
同用名
rHuCarbonic Anhydrase 4, His; Carbonic Anhydrase 4; Carbonate Dehydratase IV; Carbonic Anhydrase IV; CA4
氨基酸序列

AESHWCYEVQAESSNYPCLVPVKWGGNCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISGGGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPEDEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYFRYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQLGQRTVIKHHHHHH

分子量

Approximately 30.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 100 mM NaCl, pH 8.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Carbonic Anhydrase 4 Protein, Human (His)
目录号:
HY-P7720
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