2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3) Cathepsin
  5. Cathepsin E
  6. Cathepsin E 蛋白, Rat (HEK293, His)

Cathepsin E 蛋白, Rat (HEK293, His)

目录号: HY-P74344
产品使用指南

Cathepsin E 蛋白是一种天冬氨酸蛋白酶，在蛋白质的降解、生物活性蛋白的生成和抗原加工中起重要作用。Cathepsin E Protein参与了年龄诱导的神经元死亡通路的执行，以及兴奋毒素对谷氨酸受体的过度刺激和短暂性前脑缺血。Cathepsin E 蛋白是胰腺导管腺癌 (PDAC) 的潜在早期生物标志物。Cathepsin E 蛋白, Rat (HEK293, His) 是重组的 组织蛋白酶 E 蛋白，由 HEK293 表达，带有 C-His 标签。Cathepsin E 蛋白, Rat (HEK293, His) 全长 398 个氨基酸，分子量约为 ~42.1 kDa。

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描述

Cathepsin E Protein is an aspartate protease that plays an important role in protein degradation, bioactive protein production and antigen processing. The Cathepsin E Protein is involved in the execution of age-induced neuronal death pathways, as well as overstimulation of glutamate receptors by excitotoxins and transient forebrain ischemia. Cathepsin E Protein is a potential early biomarker of pancreatic ductal adenocarcinoma (PDAC). Cathepsin E Protein, Rat (HEK293, His) is the recombinant rat-derived Cathepsin E protein, expressed by HEK293 , with C-His labeled tag. The total length of Cathepsin E Protein, Rat (HEK293, His) is 398 a.a., with molecular weight of ~42.1 kDa.
研究背景

Cathepsin E 是一种天冬氨酸蛋白酶，也是肽酶 A1 蛋白酶家族的一员，主要表达于免疫系统、胃肠系统、淋巴组织、红细胞和癌细胞中。Cathepsin E通过水解特定肽序列位点的肽键来分解蛋白质，在蛋白质的降解、生物活性蛋白的生成和抗原加工中起重要作用。Cathepsin E 参与了年龄诱导的神经元死亡通路的执行，以及兴奋毒素对谷氨酸受体的过度刺激和短暂性前脑缺血。Cathepsin E 可能在肠化生分化为高分化腺癌的过程及树突状细胞中起作用。
种属

Rat
表达系统

HEK293
标签

C-His
蛋白编号

AAH62002 (M1-P398)
基因 ID
蛋白结构
N-term
Cathepsin E (M1-P398)
Accession # AAH62002
His
C-term
同用名
Cathepsin E; CTSE; CATE
分子量

Approximately 42.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Cathepsin E Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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