CD146/MCAM 蛋白, Mouse (HEK293, His)

The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of CD146/MCAM Protein, Mouse (HEK293, His) is 540 a.a., with molecular weight of 90-100 KDa.

CD146/MCAM 是一种多功能蛋白质，在血管组织内细胞间连接处的细胞粘附和内皮单层的凝聚力中发挥着至关重要的作用。它的表达对于促进黑色素瘤细胞和血管系统细胞成分之间的相互作用具有重要意义，可能会增加肿瘤的血行扩散。除了参与肿瘤生物学之外，CD146/MCAM 还被认为在胚胎发育过程中充当粘附分子，特别是在神经嵴细胞中。作为表面受体，它触发涉及 FYN 和 PTK2/FAK1 的酪氨酸磷酸化事件，并伴随细胞内钙浓度的短暂增加（通过相似性）。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.

Mouse

HEK293

C-His

Q8R2Y2 (V24-V563)

84004  [NCBI]

VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV

90-100 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.