1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. CD39L4/ENTPD5 蛋白, Mouse (sf9, His)

CD39L4/ENTPD5 蛋白, Mouse (sf9, His)

目录号: HY-P76317
产品使用指南

CD39L4/ENTPD5 蛋白位于内质网腔内,通过水解核苷二磷酸(包括 GDP、IDP 和 UDP),在糖蛋白加工中发挥至关重要的作用。其酶活性对于 UDP 的清除至关重要,UDP 是 UDP-Glc:糖蛋白葡萄糖基转移酶的终产物反馈抑制剂。CD39L4/ENTPD5 蛋白, Mouse (sf9, His) 是重组的 CD39L4/ENTPD5 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。CD39L4/ENTPD5 蛋白, Mouse (sf9, His) 全长 409 个氨基酸,分子量约为 ~45 KDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

The CD39L4/ENTPD5 protein is located in the lumen of the endoplasmic reticulum and plays a crucial role in glycoprotein processing by hydrolyzing nucleoside diphosphates (including GDP, IDP, and UDP). Its enzymatic activity is critical for the clearance of UDP, an end-product feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferase. CD39L4/ENTPD5 Protein, Mouse (sf9, His) is the recombinant mouse-derived CD39L4/ENTPD5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of CD39L4/ENTPD5 Protein, Mouse (sf9, His) is 409 a.a., with molecular weight of ~45 KDa.

研究背景

CD39L4/ENTPD5 蛋白通过水解核苷二磷酸表现出其酶活性,与 ADP 和 CDP 相比,GDP、IDP 和 UDP 具有显着的偏好。特别是在内质网腔内,CD39L4/ENTPD5 在清除 UDP 中发挥着至关重要的作用,UDP 充当 UDP-Glc:糖蛋白葡萄糖基转移酶的终产物反馈抑制剂。通过水解 UDP,CD39L4/ENTPD5 通过反向转运蛋白机制促进细胞质中 UDP-葡萄糖的再生,确保蛋白质重新糖基化的正向调节。这一过程对于内质网内糖蛋白的正确折叠和质量控制至关重要,强调了 CD39L4/ENTPD5 在维持与糖蛋白加工相关的细胞功能中的重要性。

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q9WUZ9 (T19-S427)

基因 ID

12499  [NCBI]

蛋白结构
N-term
ENTPD5 (T19-S427)
Accession # Q9WUZ9
His
C-term
同用名
Ectonucleoside triphosphate diphosphohydrolase 5; NTPDase 5; CD39 antigen-like 4; CD39L4
分子量

Approximately 45 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CD39L4/ENTPD5 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD39L4/ENTPD5 Protein, Mouse (sf9, His)
目录号:
HY-P76317
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