1. 重组蛋白
  2. CAR-T related Proteins CD Antigens
  3. CD4 T Cell CD Proteins Dendritic Cell CD Proteins
  4. CD4 蛋白, Rhesus Macaque (HEK293, His)

CD4 蛋白, Rhesus Macaque (HEK293, His)

目录号: HY-P77616
产品使用指南

CD4 蛋白形成表面二聚体,与 LCK 和 PTK2/FAK1 相互作用,并与 P4HB/PDI 结合。它通过 IL16 诱导 CD4 依赖性信号传导。CD4 与 MHCII α 和 β 链相互作用,增加 TCR 亲和力。CD4 的寡聚化对于稳定的 MHCII 结合和 T 细胞-APC 粘附是必要的。CD4 蛋白, Rhesus Macaque (HEK293, His) 是重组的 CD4 蛋白,由 HEK293 表达,带有 C-His 标签。CD4 蛋白, Rhesus Macaque (HEK293, His) 全长 365 个氨基酸,分子量为 45-52 kDa。

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     可免费申领三个不同产品的试用装。

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描述

CD4 Protein forms surface dimers, interacts with LCK and PTK2/FAK1, and binds to P4HB/PDI. It induces CD4-dependent signaling with IL16. CD4 interacts with MHCII alpha and beta chains, increasing TCR affinity. Oligomerization of CD4 is necessary for stable MHCII binding and T cell-APC adhesion. CD4 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived CD4 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD4 Protein, Rhesus Macaque (HEK293, His) is 365 a.a., with molecular weight of 45-52 kDa.

研究背景

CD4 蛋白在细胞表面形成二硫键连接的同二聚体,并与多种蛋白相互作用,包括 LCK、PTK2/FAK1 和 P4HB/PDI。它还与 IL16 结合,导致淋巴细胞中 CD4 依赖性信号传导。此外,CD4 与 MHCII α 和 β 链相互作用,增强 T 细胞受体 (TCR) 对肽-MHCII 的亲和力。CD4 的寡聚化,特别是通过 Ig 样 C2 型结构域 2 和 3 进行的寡聚化,对于稳定结合 MHCII 并促进 T 细胞和抗原呈递细胞 (APC) 之间的粘附至关重要。

种属

Rhesus Macaque

表达系统

HEK293

标签

C-His

蛋白编号

G7N5T8 (K26-W390)

基因 ID

/

蛋白结构
N-term
CD4 (K26-W390)
Accession # G7N5T8
His
C-term
同用名
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
分子量

45-52 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD4 Protein, Rhesus Macaque (HEK293, His)
目录号:
HY-P77616
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